| SubjectAging is a complex development process in life cycle, during which the functions of the body are deteriorated until its death. The free radical theory of aging indicates that the effect of membrane damage produced by free radicals and lipids peroxidation is one of the major reasons of aging. With the age increasing , excessive free radicals production and lower ability of elimination alternates the metabolism of body and the action of enzymes. Apoptosis may be the result of the imbalance.Phytochemicals have a strong capability of antioxidation. They can eliminate free radicals effectively, thus stave off occurring and development of aging. β - carotene and lycopen, which were studied more often, have many functions, such as antioxidation, restraining mutation, depressing the damage of nucleic acid, preventing aging and cancer. Increasing of the intake of carotenoid - rich vegetables and fruits may enhance the concentrations of carotenoid, such as |3 -carotene and lycopen, in blood and organism, so the ability of antioxidation may be advanced.There are different impacts between single antioxdant and whole vegetables and fruits. We studied the aging preventing effect in rats which were aging produced by D - galactose, using two carotenoid - rich vegetables, carrot and tomato , treated directly with feeding free. This will provide the base data for further study in human body.Materials and Methods1. Treatment of carrot and tomatoNatural grown tomato and carrot were selected as experimental materials, left the parts of availability which were washed three times with water without i-ons. After weighted up, the homogenate of carrot and tomato with 5% plant oil in cans were put in the condition of high pressure at 0. 05MPa for 10 minuets, then cooled and stored at 0 -4 against light.2. Indexes analysis2.1 The actual intake of carrot and tomato: Weighting the carrot or tomato left in the next morning to calculate the actual intake.2.2 The character of aspect: Observing the changes of aspect at any moment and weighted up the rats every week.2.3 The concentration of MDA; With the method TBA colorimetry;2.4 The activity of SOD; With the method nitrite reduction;2.5 The activity of GSH -Px: With DTNB colorimetry;2.6 Ratio of apoptosis of spleenocytes: Analyzed by PCM using propidium staining method.3. Animals feeding and treatment32 six - week health male Wistar rats were fed for one week, fallen into four groups in random( n = 8 ) : normal group ( group A) , aging group ( group B) , carrot group ( group C) and tomato group ( group D). The rats ate and drank freely. Every day, rats of group A were subcutaneous injected with saline 100ml/kg. bw at dorsal of neck; rats of group B, C and D were subcutaneous injected with D - galatose 100mg/kg. bw, at the same time, rats of group C were fed with carrot 15g, and rats of group D were fed with tomato 15g. At the 25th weekend of the experiment, we stopped the injection of saline in the group A and of D - galatose in the other groups.4. Statistics analysisAll data were analyzed with ANVON by SPSS statistic software.its1. Actual intake of carrot and tomatoEvery day, the average actual intake of carrot for every rat in carrot groupwas 13. 8g, the average actual intake of tomato for every rat in tomato group was 13.7g.2. The characters of aspectCompared with the other rats, the rats of aging group were scorcher in hair, slower in action and cachexia. From the 6th week to the 13th, the weight of rats in notmal group was lower in evidence than those of the other rats in aging group, carrot group and tomato group (P <0.05).3. The concentration of MDAAt the 10th weekend, the concentration of MDA in the serum of rats in aging group was increased obviously than those of the other rats in normal group, carrot group and tomato group (P <0. 05). At the end of the study (the 26th weekend) , the increased concentration of MDA in the liver of rats in aging group was more significant than those in the nor...
|
PDF Full Text Request