| Research Background CD44 is a kind of extracellular transmembrane glycoprotein, which normally takes part in cell-cell and cell-matrix adhesion interactions, lymphocyte activation, homing and cell migration. The Cells known to express CD44 include lymphocytic cells, monocytes cells, epitheliall cells and tumor cells. CD44 belongs to a cellular adhesive molecule. There are multiple ligands for CD44, including hyaluronate, fibronectin, laminin, osteopontin, collagen and so on. CD44 could increase the adhesion of both vascular endothelial cells and tumor cells. CD44v6 is a variant isoform of CD44. It has been confirmed that CD44 expression is related with the development, infiltration and metastasis of tumors, and involved in the development of inflammatory diseases.Retinoblastoma(RB) is a malignant tumor originated from the retinal cell of the eye, which is the most common eye cancer inchildren, and the third most common tumor overall affecting children. The incidence of retinoblastoma is rising. It now occurs in 1 out of every 15,000 live biths. Untreated, retinoblastoma is almost always fatal; therefore, early diagnosis and treatment is critical in savinglives and preserving visual function. However, there are few reports on the relationship of CD44 and RB.Proliferative vitreoretinopathy (PVR) is the most common cause for failure of retinal detachment surgery and is characterized by the formation of fibrocellular epiretinal or subretinal membranes. These membranes can contract and result in traction retinal detachment. Basic research showed that PVR is the final stage of a wound healing process. There is unknown preventative therapy, andthe current intervention but surgical removal. Based on the these considerations, it is of great significance to better understand and observe this disease. It has been reported that adhesion molecule Mac-1 and ICAm-1 are involved in the development of PVR. However, the expression of CD44v6 in the epiretinal membrane has seldom been reported.This experiment is to observe the expression of CD44v6 in RB and PVR by immunohistochemistry, to explore the relationship of it and the development of two diseases. It may give a new approach to treat, prevent and evaluate prognosis of the diseases.AIM(1) To observe the expression relation of CD44 with the development, infiltration and metastasis of retinoblastoma, and present a significant basis for malignancy and prognosis.(2) To observe the expression of CD44v6 in epiretinal membranes of PVR.METHODS(1) Six specimens normal retinas and twenty-two -specimens retinoblastomas were divided into differentiated tissues,non-differentiated tissues, the optic nerve infiltrated group ,and the optic nerve non-infiltrated group according to the pathology type, which were studied by immunohistochemical in order to investigate the expression of CD44v6.(2) Thirteen specimens epiretinal membranes of PVR obtained during vitrectomy were divided into grade C and grade D according to the grade of PVR, which were studied by immunohistochemical in order to investigate the expression of CD44v6 .RESULTS(1) The positive staining of CD44v6 was located in the cytoplasm of tumor cell. Expression of CD44v6 was noted positively in 16 of 22 specimens of retinoblastomas, the positive rate was 72.7%. CD44v6 was 9/14 positively expressed in RB with differentiated tissues, the positive rate was 64.2%, which was 7/8 in non-differentiated tissues, the positive rate was 87.5% ( P>0.05 ). CD44v6 was 12/13 noted positively in RB with optic nerve infiltrated group, the positive rate was 92.3%, which was 4/9 in optic nerve non-infiltrated group, the positive rate was 44.4%( P< 0.05 ). CD44v6 was weak staining in 1 case normal retina, the positive rate was 16.6%.(2) CD44v6 was positively expressed in 10 of 13 cases epiretinal membranes, the positive rate was 76.9%. The positive staining of CD44v6 was located in the cytoplasm of ce...
|
PDF Full Text Request