The Experimental Study Of Autogeneic Graft Of Parallel Bi-Vein With Valves

Objective To establish the animal model of autogeneic graft of parallel bi-vein with valves and study the feasibility of the transplantation and seek a new surgical method for venous valve reconstruction in patients with chronic venous insufficiency. To search for the experimental and theoretical foundation for the clinical application of the new technique.Methods 24 New Zealand rabbits were divided into experiment group and control group randomly. The parallel bi-vein with valves autogeneic graft animal model was established in experiment group and the vein which was incorporated with external and internal jugular vein autogeneic graft in control group by microsurgery. The hemorheologic and hemodynamic changes were examined by real time Color-duplex scanning and phlebography. The transplanted vascular segments were perfusion-fixed in situ and dissected, then observed by light microscope and scanning electron microscope 4 weeks postoperatively. Results The animal models of autogeneic graft was established successfully in all the 24 rabbits. One was died of intraabdominal hemorrhage in control group 12 hours postoperatively because of the over dose of heparin during operation. Only 1 rabbit per group was obstructed in the 4 weeks because of the compression of the adhering zone of peritoneum, which was confirmed by anatomy. And the rest remained patency which kept favorable configuration and steady blood stream with laminar flow and the results observed by light microscope and scanning electron microscope revealed that there was no injury of endomembrane and no thrombosis in the transplanted fragment. Conclusion The autogeneic graft of parallel bi-vein with valves by microsurgery may be a feasible and promising technique of deep venous reconstruction for patients with chronic venous insufficiency.