The Experimental Comparison Of The Medicine Influence On Lens Sodium-Pump

OBJECT: To investigate the influence and mechanism of several kinds of medicine on lens sodium-pump, to furnish the theoretic foundation and experimental evidence for further researching on cataract pathogeny and exploring on its drug therapy. METHODS: Randomly devide 56 Wistar rats into 7 groups , each group are injected by a special medicine.Kill the rats and take out their lens to observe the cells morphological changes under the light microscope , to explore the ultramicroscopic structure alteration under the electron microscope and examine the expression condition of αsubunit of sodium pump on mRNA level by means of RT-PCR. RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1,α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1,α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1,α2 and α3-isoform are all altered slightly and haven't significant meanning. CONCLUSION:Each medicine used in the study totally has obivious effect on lens sodium-pump. Either on the molecular and cellular level or on the histological and organic level , they all have their distinctive demonstration. 1.Ouabain,Digoxine,DMSO decrease the expression of αsubunit of sodium pump on mRNA level , the cell morphology and ultramicroscopic structure demonstrated destructive effect,it indicates the cause of cataract of the medicines.2.D-thyroxine,Amphotericin B,Vitamin E increase the expression of αsubunit of sodium pump on mRNA level ,from the demonstration of the cell morphology and ultramicroscopic structure ,it indicates the medicines have protective or anti-distructive effect on lens.3.The medicines have isoform-specific action on lens sodium-pump,it indicates exploitting isoform-specific medicine has a promising clinical application.4. Lens sodium-pumps are inhibited probably lead to cataract, so in clinical medication, especially to the cardiovascular doctor, while he is using the sodium-pump inhibitors, he should pay more attention to adjust the dosage and duration of the drug therapy. 5. To creat the animal model of cataract through inhibiting lens sodium-pump, whether it can be a kind of classical method or not, it is worthy of further research.