Expression And Significance Of Tumor Suppressor Gene-ING1 MRNA In Bladder Carcinoma

Objective To explore the expression of ING1 mRNA in bladder carcinomas, and evaluate the relationship between the expression level and the progression of tumor. Methods The specimens from 38 patients including cancer tissues and normal tissues around tumors was detected for their expression levels of ING1 mRNA by RT-PCR. Results We found the positive expression of ING1 mRNA in all normal tissues around tumors. A significant decrease in ING1 mRNA was found in 32 out of 38 bladder carcinomas(32/38,84.2%),and 2 specimens were found no expression. The expression level in tumors was different significantly to that in normal tissues(P<0.001). It showed that repression of ING1 mRNA expression might be correlated to the stage,grade and metastasis. With the decrease in ING1 mRNA expression, the prognosis markedly aggravated. The expression of ING1 mRNA in Ⅲ-grade group (0.355±0.230)was obviously lower than the expression in Ⅱ-grade group(0.608±0.143). The expression of ING1 mRNA in Ⅱ-grade group was obviously lower than the expression in Ⅰ-grade group(0.926±0.143)(P<0.0001). And The expression of ING1 mRNA in T3﹢T4 group(0.377±0.240)was obviously lower than the expression in T2 group(0.665±0.194). And the expression of ING1 mRNA in T2 group was obviously lower than the expression in Ta﹢T1 group(0.899±0.164)(P<0.0001). And the expression of ING1 mRNA in lymphnode-positive group(0.397±0.197)was obviously lower than the expression in lymphnode-negative group(0.735±0.259)(P<0.001). Also it showed that the expression level was not correlated to gender and age . The two specimens with no expression distribute in T3,T4 and low-grade cancer. Conclusion The repression of ING1 mRNA expression might be negatively correlated to the stage,grade and metastasis of bladder carcinoma. Down-regulation of ING1 may play an important role in the oncogenesis and development of bladder carcinomas. It may be used as an candidate indicator for malignity-judgement,diagnosis and prognosis of bladder carcinomas. More investigation would be need in the biological and clinical significance of ING1.