Expression And Meaning Of Vascular Endothelial Growth Factor And Thrombospondin In The Ectopic Endometrium Of Women With Endometriosis

Endometriosis (EM) is a reprodrctive disease characterized the presence and growth of ectopic endometrial tissue at sites outside the uterus. With the incidence in the general population gradually being raised in the past few years, EM has been considered to be one of the most commonly encountered diseases of gynecology besides myoma of uterus. Although the histogenesis and recurrence of EM and associated etiologic factors have been extensively studied and there are numerous theories proposed for the origen of EM, no single theory can account for the location of the ectopic endometrium in all cases of EM, Factors that allow the implantation and propagation of EM, still elude gynecologist EM is common benign condition , though its pattern of growth is similar to malignant disease. It is difficult to be treated with medicine because of obvious side effect and high recurrence rate. Recent findings supports that the form of the ectopic endomtrium is depend on the neovalscualization , and the balance of stimulator and inhibitor is much more important in this faction. However, there are not systematic studies on it yetVascular endothelial growth factor (VEGF) is an important angiogenic factor which can promote the neovalscualization. Urokinase-type plasminogen activator (uPA) is an important angiogenic factor also which can promote the neovalscualization when there is VEGF. Throm bospondin(TSP) and plasminogen activator inhibitor-l(PAI-l) are angiogenic factors which can inhibit the neovalscualization.The purpose of this study is to explore the effect and the meaning of these angiogenic factors in etiology of EM, and also provide experimental data for applying angiogenic inhibitor factor in treating EM.In this study, first, we investigated the expression of VEGF, uPA/TSP, PAI-1, F-VIII in 30 cases ectopic and eutopic endometrium with EM, by means of immunohistochemical analysis. At the same time, we investigated the expression of VEGF, uPA, TSP, PAI-1, F-VIII in 14 casesendometrium without EM, and then compared result each other. Second, we investigated the expression of VEGFmRNA by means of in situ hybridization analysis in 30 cases ectopic and eutopic endometrium with EM, and compared with endometrium of 14 cases without EM Result: the expression of microvessel count (MVD),VEGF, VEGFmRNA, uPA in ectopic endometrium was high compared with EM eutopic endometrium and no EM endometrium (P<0.05). The highest MVD expression wasfound in stage I -II EM There were no significant difference between theexpression of TSP and PAI-1 in EM ectopic and eutopic endometrium and no EM endometrium . There is an positive relationship between the expression of VEGF to MVD, and the expression of VEGF to uPA. Conclusions: the angiogenic of EM ectopic endometrium is abnormal which is very important in etiology of EM There are imbalance between stimulators and inhibitors, for example, VEGF and TSP, uPA and uPA-I, VEGF and uPA have additive action in faction of angiogenic and the formation of EM. Therefore we supposed that applying angiogenic inhibitor factor in treating EM may be an effective means which can improve effect of diverse therapies.