Experimental Study On The Therapeutic Mechanism Of Shen-An Chongji (SACJ) Treating Nephrotic Syndrome

Objective: To study the therapeutic mechanism of SACJ treating nephritic syndrome.Methods: Nephrotic syndrome(NS) rat models were induced by tail intravenous injection with adriamycin(ADR). The experiment rats were randomly divided into SACJ group, Prednison group, SACJ combined with Prednison group, the model group and normal control group. 2 weeks after the models being made, the admistration of drugs started and lasted for 8 weeks. The content of 24- hour urinary protein, serum albumin, (Alb), BUN, Scr , blood lipid level, activities of lipoprotein lipase (LPL),morphology of renal histology, collagen I and collagen â…¢ were detected . Results: 1.The contents of 24-hour urinary protein, lipid level increased and serum albumin level ,activities of LPL decreased significantly in model rats compared with normal rats (p<0.01).The number of mesangial cell, the area of mesangial matrix and the surface area rate of collagenâ… and collagen â…¢ of model rats were obviously higher than those of normal rats(p<0.01).2. The contents of 24-hour urinary protein and lipid level of all treated group rats were obviously lower than thoseof model group rats (P <0.05or p<0.01). Serum albumin level and activities of LPL of all treated group rats were obviously higher than those of model group rats (p<0.05 or p<0.01).The number of mesangial cell, the area of mesangial matrix and the surface area rate of collagenâ… and collagen â…¢ were significantly reduced in all treated group rats compared with model group rats (p<0.05 or p<0.01).3.There were markedly differences of all test parameters between SACJ group and Prednison group which indicated the therapeutic effect of SACJ was better than that of Prednison for nephritic syndrome. Conclusion: SACJ decreased the contents of 24-hour urinary protein and lipid level, increased Alb level and activities of LPL, inhibited the proliferation of mesangial matrix and mesangial cell and reduced the deposition of collagen â…  and collagen â…¢.So it relieved the lesion of kidney owing to the pathologic agents. SACJ could fundamentally inhibit the ECM accumulation of ADR rats and slow the processes of glomerulosclerosis and renal interstitial fibrosis...