Study On The Expression Of Cell Adhesion Molecules And Cytosolic Ca～(2+) In Patients With Coronary Artery Disease

Coronary artery disease (CAD) has been the major death cause in the world since 1960's Last century. Studying it's etiology , pathophysiology, diagnosis, treatment and prevention has become an important topic in clinical medicine. In recent years, the role of cell adhesion molecules (CAMs) in the pathogenesis of CAD has gained increasing attention. In this study, we evaluated changes in leukocyte counts and expressions of CAMs (CDllbN CD^ CD62L) in peripheral blood leukocytes in patients with CAD, such as acute myocardial infraction (AMI), unstable angina pectoris (UA), stable angina pectoris(SA). In addition, The changes in PMN cytosolic Ca + was determined, which is highly related to the activation of neutrophils. The results of this study will provide useful information for in-proving our understanding of the pathgenesis of CAD and its treatment.The expression of CAMS in leukocytes and PMN cytosolic Ca2+ were examined using flow cytometry (FCM). The result shows that leukocyte counts, mainly neutrophil counts in peripheral blood and expression of CDllb^ CD18 on neutrophils in the CAD patients were markedly higher than those in control group (p<0.001). The expression of CD1K CD 18 on neutrophils in patients with AMI and UA were significantly higher than those in patients with SA Cp<0.01). Down-regulation of CD62L on neutrophils, up-regulation on PMN catabolic Ca2+ were found in patients with CAD (p<0.001). We also found the positive correlations between the expression of CDllb> CD 18 and PMN cytosolic Ca2+, the negative correlations between the expression of CD62L and PMN cytosolic Ca2+(r=0.595, 0.563, -0.656).These results showed: Leukocyte plays an importent role in the progression and pathogenesis of CAD. The increase of expression of CD11b, CD 18 in peripheral blood leukocytes in patients with CAD indicated the activation of neutrophils, the increase of the adhesion between neutrophils and endothelial cells. The increased degreeexpression might be related to the types of myocardial ischemia. The down-regulation of CD62L is the signal for the activation of neutrophils. CD62L and PMN cytosolic Ca2+ are also associated with occurrence and progression of CAD.