Suppressive Effects Of Taxol On Vitro Invasion Of Adenoid Cystic Carcinoma And Elementary Research Of Mechanism

Object To investigate the effect of taxol on vitro invasion, expression and activity of gelatinase (MMP~2, MMP-9) of human adenoid cystic carcinoma(ACC). Methods Highly mestastatic ACC cell line(ACC-M) was adopted, which was treated with various concentration of taxol (0.1, 0.06, 0.01mg/L). Using MTT technique, to assay the ACC-M cell-ECM adhesion and the effect of taxol on viability of ACC-M. ACC-M cell-ECM invasion and migration were evaluated using modified Boyden chamber. Using technique of colorless lot, to assay the activity of gelatinase (MMP-2, MMP-9) in culture supernatant of ACC-M. The contents of MMP-2 and MMP-9 in culture supernatant was detected with ELISA. In addition, to evaluated the suppression of taxol on expression of MMP-2 and MMP-9 of ACC-M by immunocytochemistry (LSAB method). Results It was found that taxol (0. 1-0.01mg/L) inhibited ACC-M to adhere and migrate by 13. 3%-41. 2%, 22.0%-72.3%, respectively. At concentration of 0.1mg/L, 0.06mg/L and 0.01mg/L, taxol suppressed ACC-M to invade the ECM by 78.4%, 64.9%, 42.3%, respectively. In addition, the effect of taxol on viability of ACC-M was negligible. The diameter of each colorless lot indicated the activity of gelatinase.It was found that taxol(0. 1-0. Olmg/L) inhibited activity of gelatinase by 66. 6%-38. 8%. At taxol concentration of Omg/L, 0.01mg/L, 0. 06mg/L and 0. Img/L, the contents of MMP-2 in culture supernatant were 581pg/105 cell, 397 pg/105 cell, 187 pg/105 cell, 86 pg/105 cell, the contents of MMP-9 were 633pg/105 cell, 273 pg/105 cell, 208 pg/105 cell, 127 pg/105cell. In addition, it was showed that positive indexes of expression of MMP-2 and MMP-9 of ACC-M were decreased remarkably following the increasing of taxol concentration. Conclution Taxol can significantly inhibit vitro invasion of ACC-M. In addition, taxol can result in the down-regulation of expression and activity of MMP-2 and MMP-9. Therefore, It can be deduced that taxol can exert multiple suppressive effects on the invasion and mestastasis of adenoid cystic carcinoma.