| Liver transplantation (LTx) has become the most effective treatment for the end-stage liver diseases. Up to now, the long term survival rate has reached 70 %, but 5%-10% of the patients have to accept retransplantation for primary non-function (PNF), the most serious complication after transplantation . At present, it is believed that the cold preservation and reperfusion injury is the major cause of PNF, and the damage of sinusoidal endothelial cells (SEC) directly leads to the injury during this period.It is of great value to protect liver graft and reduce SEC injuries during cold preservation, the long period during transplantation, and then to avoid PNF after transplantation..According to pharmacology, Ginkgo biloba extract (Ginaton) can specially protect ATP synthesis against anoxia/reoxygenation injury by scavenging the superoxide anion generated by mitochondria. Ginaton also can improve hepatic microcirculation in the rat after warm ischemia. Whether Ginaton can protect the cold preservation and reperfusion injury during liver transplantation , to be investigated. ObjectiveIn this study, whether both the function and the structure of SEC could be protected during cold preservation by adding a certain concentration of ginkgo biloba extract (Ginaton) to Euro-collins solution, was investigated. MethodsOne hundred and nineteen Wistar rats were randomly allocated into 9 groups.There were seven rats in the normal control group (N). The rest rats were allocated into eight groups (fourteen rats in each group, 7 as donors and 7 as recipients) as follows: experimental control group 1 (C1), experimental control group 2 (C1), experimental control group 3 (C3), experimental control group 4 (C4), experimental group 1 (E1), experimental group 2 (E2), experimental group 3 (E3), and experimental group 4 (E4). For groups C1 and E1, groups C2 and E2, groups C3 and E3, groups C4 and E4, the cold preservation time was 3, 6, 8, 12 hours, respectively. Liver grafts in each experimental groups were flushed with and preserved in 4 Euro-collins solution containing 0.2g/L Ginaton. Liver grafts in each experimental control groups were only flushed with and preserved in 4* Euro-collins solution. Then orthotopic liver transplantation was performed. At 15 min, 30 min, and 60 min after portal vein reperfusion, blood samples were obtained to determine the levels of ALT, AST, ET, and HA. One hour after portal vein reperfusion , grafts samples were fixed by 2.5% glutaraldehyde for electronscopy observation. During operation, non-hepatic time and surgical operation time for recipients were recorded. In normal control group liver transplantation was not performed and the samples were obtained with same methodas used in other groups. Data were presented as x±s or, when indicated, number andpercentage. Statistical analysis were performed with SPSS 10.0 for WINDOWS statistical package. Data were analyzed statistically with t test, one-way analysis of variance (ANOA), or repeated measure analysis. Correlation was assessed by the method of least squares. P value <0.05 was considered significant. Results1. Non-hepatic time, and surgical operation time for experimental control groups and experimental groups had no discriminating (P>0.05).2. At 60 min after portal vein reperfusion, the level of ALT and AST in both experimental groups (E1,E2,E3,E4) and experimental control groups (C1, C2, C3, C4) were significantly higher than that in normal control group (P<0.05); the level of ALT and AST in experimental groups (E1,E2, E3, E4) were significantly lower than that in corresponding experimental control groups (C1, C2, C3, C4) (P<0.05); within theexperimental control groups (C1, C2, C3, C4), the level of ALT and AST was increasing in the sequence of d, C1, C3, C4 (P<0.05); and within the experimental groups (E1, E2, E3, E4), the level of ALT and AST was also increasing in the sequence of E1, E2, E3,E4, but the degree was lower than that of the experimental control groups (C1, C2, C3, C4) (P<0.05).3. At 15... |
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