| Background and objective According to the conventional idea, the gastric movement was controlled by vagus nerve. The gastric peristalsis and empty function would be disorder after vagotomy. So it was necessary to add to pyloroplasty after vagotomy. However, it was found that the gastric emptying disability didn't happen in the most patients performed upon proximal subtotal gastrectomy without added pyloroplasty. The conventional idea was doubted by surgeons. But the compensatory mechanism of gastric peristatsis and emptying after vagotomy is still not clear.In order to explore the compensation mechanisms of gastric movement following the vagotomy, the models of vagotomy, vagotomy adding pyloroplasty and proximal subtotal gastrectomy were made in dogs, and the changes of electrogastrograms and interstitial cajal cells were investigated.Methods Twenty eight healthy adult dogs were divided randomly into 4 groups (7 each group). The celiotomy was performed in group A, vagotomy in group B, vagotomy adding pyloroplasty in group C, proximal subtotal gastrectomy in group D, and the electrodes were embedded at the gastric corpus and antrum in all groups. The dogs were bred for 3 months after operation. The changes of gastric electric activity were recorded at preoperation, 1 week, 1 month and 3 month after operation respectively. The histological changes of gastric tissues were observed at 3 month after operation, and the expression of c-kit protein was detected in the gastric specimens by immunohistochemistry stain. Results 1. Clinical observation During 3 months after operation, the clinical situation were similar to that before operation in group A. The dogs began eating at 2-3 day after operation and their appetites were almost same as that before operation. The body weight didn't obviously changed in 3 months after operation in all dogs. In group B, one dog died at 5 hours after vagotomy, the others were well survival. Mild anorexia symptom appeared only in one dog in the early stage. They began drinking in 2-3 day, and eating after 3-4 day.Their appetite was similar to preoperation after 2 weeks. The body weight didn't obviously changed in 3 months after operation in all dogs. The clinical situation in group C was similar to that in group B. One dog in group C died of the infection at the 6th day after operation. No obvious gastric retention was found in both group B and C. In group D, 2 dogs died of infection, 5 dogs recovered slowly, nausea and vomit were observed in 2 dogs for 3 days after operation. Anorexia was observed, and ameliorated till 2 weeks after operation in group D. Nevertheless, there was no obvious changes of body weight after 3 months in group D.2. Changes of gastric electrical activity The wave form resembled to sinusoidal wave, amplitudes of slow wave were coincident in all the dogs in group A. Abnormal gastric electrical activity occurred occasionally. The wave form was also sinusoidal wave in group B and C. Bimodal wave appeared in those two groups. There were no obvious differences on the frequency of waves, amplitude and transmission speed of electric waves between group B and group C. The frequency of slow waves was faster in those two groups than in group A. Amplitude and transmission speed was lower in those two groups than in group A. Abnormal gastric electrical activity was found frequently in group B and C. The amplitude and transmission speed of slow waves were similar to those before operation in group B and C after 5 weeks. The frequency of slow waves was still faster in group B and C than in group A, abnormal gastric electrical activity occurred by chance. In group D, the wave form was also sinusoidal wave after operation, but a lot of dentate wave overlapped on the spike potential, those waves form showed irregular, and the amplitude was incoincident. The abnormal gastric electrical activity was found frequently in group D. The frequency of slow waves was faster in group D than in group A, and similar to group B and C. The amptitude and transmission sp...
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