| Objective: Cancer is a kind of fatal disease with high death rate. There are many ways to treat cancer including application of anti-tumor ingredients of mushroom which has little adverse reaction and remarkable curative effects.LetinusdodesC91-3, whose fermentative liquid has notable effects on anti-tumor and anti-bacteria, is a special fungus which has been screened for many years .Based on fermentative liquid of letinusdodesC91-3,we extracted ingredient of proteins(LFP91-3).We studied anti-tumor effect of LFP91-3 and its mechanism which could be developed as a theory basis for anti-tumor drugs.Method: 100 inbred male and female BALB/C mice bearing H22 and U14 tumor were divided into 5 groups equally and randomly at 10 mice per group: NS control group, CY(cyclophsphamide) treatment group, lentinusdodesC91-3 mycelium's fermentative liquid protein(LFP91-3) 1 treatment group,LFP91-3 Ⅱ treatment group,LFP91-3 Ⅲ treatment group.24 hours after implanted with tumor, NS was injected into mice of NS group intraperitoneally with NS 1ml per mouse once every day for 5 consecutive days. CY 0.4mg, LFP91-3 50ug, LFP91-3 100ug, LFP91-3 150ug were injected into mice of CY group, LFP91-3 I group, LFP91-3 Ⅱ group,LFP91-3 Ⅲ group respectively in the same way. The therapeutic effect was determined by life span. Another 50 inbred male and female BALB/C mice bearing H22 substantial tumor under right axilla were divided into the same 5 groups. We injected into the same liquid under right axilla' epidermis with thesame dose, 1ml per mouse once every day for 10 consecutive days. All mice were killed 13 days after bearing tumor. The therapeutic effect was determined by means of tumor weight and pathological examination of tumor tissue. This part aims to study the therapeutic effect of LFP91-3 and its mechanics in vivo.H22, U14, S180 cell lines were respectively cultivated in vitro. LFP91-3 (5mg/ml, 10mg/ml, 15mg/ml) with different concentrations were added into cell culture liquid. The inhibition rate was determined by MTT assay at different time points. Different concentrations of LFP91-3 were also added into H22, U14 cell culture liquid. Cell cycle and apoptosis were analyzed by flow cytometry. Microscope was used to study the cell morphology after H22 cells were treated by LFP91-3.DNA of H22 cells was extracted after the treatment of LFP91-3 to observe the changes of cells' DNA. This part aims to study the in vitro anti-tumor effect of LFP91-3 and its mechanism.Results:1.LFP91-3 could prolong the life span of mice bearing tumor, inhibit the growth of tumor.LFP91-3 could prolong the life span of mice bearing H22 tumor ,and the life span of every LFP91-3 groups was22.90±2.34d, 27. 40±2. 55d and31.90±4.02d,which was longer than that of NS group(16. 60±1. 56d) (P<0.05); the life span of LFP91-3 Ⅱ group, LFP91-3 Ⅲ group were also longer than that of CY group (21. 90±1. 73d) (P<0.05). LFP91-3 could prolong the life span of mice bearing U14 tumor. The result of life span was similar to the group of H22 tumor. LFP91-3 could suppress the growth of substantial tumor, the inhibition rate was 50.37%, 54.58%, 74.13% in LFP91-3 group and 39.55% in CY group. Each LFP91-3 group and CY group have higher inhibition rate than that of NS group(P<0.05).Each LFP91-3 group has higher inhibition rate than CY group(P<0.05)2.LFP91-3 inhibited the proliferation of cells by inducing apoptosis and arresting tumor cells at S phase. LFP91-3 could inhibit the proliferation of H22, U14 and S180 cell lines. This effect was time- and concentration-depending to a certain extent. H22's ratio of G0/G1 phase may decrease from 73.6% to 64.7% and the ratio of S phase may increase from 12.6% to 28.2%, and cell cycle stops at S phase.U14 cell line has a similar result to H22 cell line, and the ratio of G0/G1 may increase from 34.6% to 43.8%.Cellcycle also stops at S phase. After H22 and U14 cells were treated by for 24 hours, LFP91-3 induced apoptosis. The apoptotic rate increased as time went by. Characteristic apoptotic bodies cou...
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