| Objective To investigate the effects and related mechanism of Octreotide during ischemia-reperfusion injury (I-RI) associated with whole pancreaticoduodenal transplantation (WPDA) in rats. Methods The homologous Sprague-Dawley rat model of heterotopic whole pancreaticoduodenal transplantation was used. After in situ perfusion and 2 hr of preservation, the level of nitric oxide (NO) products (nitrite plus nitrate) and the activity of superoxide dismutase (SOD) in serum were determined after 3hr and 6hr of reperfusion. The changes of histology in graft and the expression of inducible nitric oxide synthase (iNOS) in pancreas were dectected by H&E stain and immunohistochemistry, respectively. Meanwhile the influence of Octreotide and Octreotide plus nitroglycerin (NTG) on NO and SOD also were observed. Results There were significant increase in the level of endogenous NO products and decrease in the activity of SOD at 3hr after reperfusion contrasted to the group of sham transplantation (p<0.01). The difference was more significant at 6hr after reperfusion. The two index of serum can be reversed after the administration of Octreotide. The degree of changes has significant difference from the group of simple transplantation. Octreotide could also improve the lesion in grafted tissue and inhibit the expression of iNOS. The changes above mentioned could be re-reversed on the basis of the group of Octreotide when the NTG was added to perfusate and perfusion solution andpreservation at 3hr after reperfusion. However, the phenomenon as previously described couldn't be seen at 6h after reperfusion. At this time, there were no significant difference between the two groups of Octreotide and Octreotide plus nitroglycerin. Conclusion Octreotide could significantly inhibit the expression of iNOS and the level of NO, increase the activity of SOD at the same time. It has the protective effect on ischemia-reperfusion injury associated with pancreas transplantation in rats.
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