Experiment On The Type 2 Diabetes Induced By Emotional Stress And The Interference Of Chinese Herb On It

ObjectiveThe research is on the basis of the DM caused by many factors to discover the mechanisms of emotion causes on DM onset and the regulatory action to rats of composite formula Xinshen NO.5 which has the efficiency of regulating Qi lowering rebellious qi and dispersing accumulation.Methodon the basis of the theories of TCM about emotion caused diseases and the modern physiological-psychological medicine, the rat models of DM disease pattern were made by abdominal injecting little dosage of STZ and imposing psychophysiological experiment stimulation (crotation Restraint and crowding) simultaneously. The experiment animals were divided into five groups: normal group stress group STZ group stress+STZ groop and treatment group by Chinese herbs. Each group was checked the level of blood glucose serum insulin serum glucago cAMP and the transcriptional level of mRNA of insulin.And at the same time we observed the change of pancreas histomorphology and macro-performance.Result1 .Apparent and persistent blood glucose level increasing and serum insulin level decreasing were observed in the rats injected with STZ under emotional stimulation, the macro-performance the center check time crossing-check number . erecting number change were caused by the emotional stress. Compared with the other groups, the difference of these index were obvious(p<0.01or p<0.05). All those indicate that the rat model was accorded with the Qi-qi disorder syndrome type of DM.The level of blood glucose of treatment group by Chinese herb decreased and the level of serum insulin increased. And the change of the macro-performance of treatment group by Chinese herb is considerably light(p<0.01or p<0.05).2.Emotional stimulation can make the STZ rats' serum glucagon level and the cAMP level of liver increased. The STZ plus stimulation group have the highest level (p<0.01orp<0.05).3.The transcriptional level of mRNA of insulin is decreased by the emotionalstimulation especially the STZ+stress group rats have the lowest level (p<0.01 or p<0.05). The treatment group level is added.Conclusion1.Emotional stimulation as an important pathogenic factor can induce DM. The model has the features of high level of blood glucose serum glucagon cAMP and low transcriptional level of mRNA of insulin .The onset is related with the change of neurosecretion system induced by emotional stimulation.2.Emotional stimulation can induce behavior disorder which reflects the features of Qi-ji disorder syndrome.3.Composite formula Xinshen No.5 can improve the action of neurosecretion system and the behavior reflection of rats obviously.