Study Of The Expression Of Integrin β₃ And Its Ligand In Cycling And Early Pregant Endometrium

Objectives To profile the changes in integrin B 3 and its ligand osteopontin expression in cycling and early pregnant endometrium and explore their relationship with primary unexplained infertility.Methods Integrin B 3 and osteopontin mRNA were detected in 65 normal cycling women during different menstrual phases or early pregnant women, and in the endometria from 20 cases with primary unexplained infertility by RT-PCR semi-quantitatively. With reg'ard to the infertile, progesterone serum concentration was quantified on the same day as endometrial sampling. Immunohistochemisty was also adopted to detect the expression of integrin B 3Results 1 Relative values of integrin B 3 mRNA were zero in both proliferative and early luteal endometrium, and 0.95 ±0.03 in midluteal phase, 0.94 ±0.02 in late luteal phase, 1.05 ±0.01 in early pregnant phase; Values in middle and late luteal endometrium were significantly different from those in early pregnant endome-trium(P<0.05). xpression of osteopontin mRNA very low in proliferative and early luteal endometrium. And it expressed primarily in middle, late luteal and early pregnant endometrium. The relative values were 0.21±0.04, 0.43± 0.05, 2.82± 0.15, 2.79±0.03, 2.05 ± 0.04, respectively. Values in middle and late luteal endometrium were higher than those in proliferative, early-luteal and early pregnant endome-trium(P<0.05), but no difference between proliferative and early luteal endome-trium(P>0.05).(3)Relative values of integrin P 3 and osteopontin mRNA in endometria from 20 patients with primary unexplained infertility were 0.47 ± 0.12 and 2.74±0.11, respectively. Compared with the normal midluteal level, the former difference was significant(P<0.01),but the latter was no significance(P>0.05). And by linear correlation analysis, we found there was no significant correlation between the values of integrin B3 and osteopontin mRNA (r=-0.1011,P>0.05).4Integrin B3 protein were negative in proliferative and early luteal endometrium, but were positive in midluteal to early pregnant endometrium. Semi-quantitative detection found the A-value were zero in both proliferative and early luteal endometrium,and 0.059955 ±0.0012120 in midluteal phase, 0598545+ 0.001124 in late luteal phase, 0.069075±0.004125 in early pregnant phase; Values in middle and late luteal endometrium were significantly different from those in early pregnant endometrium(P<0.01), but there were nodifference between those in middle and late luteal endometrium(P>0.05).5Compared with normal women, integrin 3 3 failed to express in the midluteal endometrium from women with unexplained infertility immuhistochemically. And there was great relativity between the expression of integrin 3 3 protein and mRNA(P<0.01).Conclusions Our findings were that integrin 3 3 were highly expressed in the implantation window of endometrium; It may play important roles in the process of implantation and decidualization. It might be a new liable marker of uterine receptivity. Abnormal endometrial integrin 3 3 expression might be the major cause of unexplained infertility. And osteopontin was relatively high in the midluteal endometrium. Its abnormal expression might have no significant relevance to such infertility.Postgraduate He Ronghuan Directed by Ding Ping Liu Peiqiu...