The Experimental Study On Small Intestinal IR-induced Remote Organ Second Injury

It might be a series of small intestin tissue pathophysiological processes such as swelling and destruction of vascular endothelial cell, prodution of oxygen-derived free radidicals, microvasoconstriction, assembling of platelet and neutrophil, reduction of blood flow after small intestine IR. The change of oxygen-derived free radidicals may active correlative enzyme system and apoptosis gene, induce cell apoptosis then result in morphologic tissue injuries. Researches have shown that small intestine IR not only injure bowl itself but also induce remote organ second injuries. Via building small intestine IR modles, we exame the concentration of NO and SOD in the blood, observe the expression of Bax, bcl-2, P53 and the change of ultrastructure in the remote organ of the lung, liver, heart and keydney to study IR-induced remote organ second injuries and to protect remote organ from IR-induced second injury for various reasons.AbstractAIM: To study the change of concentration of NO and SOD in the blood, the expression of Bax, bcl-2, P53, the change of ultrastructure in the remote organ of the lung, liver, heart and keydney and try to find out the small intestine IR-induced remote organ second injuries of the rat. The change of ultrastructure was observed by transmission electron microscopy.METHODS: To make models of ischemia-reperfusion of small intestine at 0, 30min, 1, 2h, 1, 3, 7d after reperfusion, the concentration of NO, SOD in the blood was examed, the expression of Bax, bcl-2, Psa in the remote organ was observed by the immunohistochemical SP method. Remoe organ ultrastructures were observed by transmission electron microscopy.RESULTS: The concentration of NO increased apparently after I/R 0 minute, but decreased significantly after I/R 2 hours, then increased gradually to a peak after 7 days. For SOD, the concentration decreased after I/R 0 minute, increased significantly after I/R 2 hours, and decreased gradually to the lowest level after I/R 7 days. The positive cells were observed. The ratio of positive cells of Bax, bcl-2, P53 increased after I/R 30 min, the ratio of positive cells of bcl-2 was higher than that of Bax (p<0.01) , after I/R two hours it decreased apparently, then increased after I/R seven days, and the ratio of Bax was higher than that of bcl-2 (p<0.01 ) .Remoe organ ultrastructures were serious injured observed by transmission electron microscopy such as chromatin concintration and mitochondriadisorder.CONCLUSION: The change of concentration of NO and SOD in blood, the expression of positive cells of Bax, bcl-2, P53 as well as the change of Remoe organ ultrastructures show that it might cause apoptosis and injuries in the remote organ after IR of small intestine of rat.