Synthesis Of New Material Chitosan-g-PVP And The Application In Keratoprosthesis

This experiment aimed to confirm whether man-made grafted chitosan scaffold is appropriate scaffold for keratocyte culture of tissue engineering.Chitosan is partially N-deacetylated chitin, and chitin is a linear homopolymer of 1,4 β-linked N-acetyl-d-glucosamine. a natural biopolymer available in plenty which is obtained from the cuticle of the marine crustaceans such as crabs and shrimps .Chitosan have neen widely investigated for the last two decades on its biomedical applications.since chitosan can be hydrolyzed by lysozyme,it is also one of the biodegradble polymers in narure.furthermore the degraded products of chitosan are nontoxic,nonimmunogenic and noncarclnogenic. Chitin and chitosan have been tried out as drug carriers because of their biocompatibility and biodegradability. Chitosan and some of its complexes have been studied for use in a number of biomedical applications .Both chitin and chitosan have many useful and advantageous biological properties in the application as a wound dressing, namely biocompatibility, biodegradability, hemostatic activity, anti-infectional activity and property to accelerate wound-healingPVP is a kind of nontoxic and water-solubility macromoleculewhich has some excellent properts such as solubility, chemical stability, membrane formation and so onSince chitosan, chemically, has both reactive amino and hydroxyl groups that can be chemical modi.cations and, physically, is relatively easy to manipulate different pore structures , it has a high potential in applications of tissue engineering. We modified the chitosan by grafting PVP onto it to get a new biomaterials with better biocompatibilityMethods for estimating the degree of substitution by elemental analysis were carried out.The biocompatibility of such grafted chitosan material was evaluated preliminarily in vitro by the culture of keratocyte. Results proved that chitosan is a promising biomaterials for tissue engineering.