Alcohol Effect On Apoptosis Of The Ovary And Granule Cell In Rat

The basis of alcohol is ethanol. which is a commonly bringing on abnormality matter, and have cellular toxicity action heavily, and can lead to many kinds of cellulars apoptosis. Kissing the cup excessively for long time can break the anthropic procreation system seriously, and have distinctness damnification action for germ cell.it is reported that alcohol have develop toxicity action for ovary, and fair sexes who drink much effect can have ovarian functional obstacle. The ovary apoptosis is mostly granule cell apoptosis.At present the disquisition of apoptosis of follicular cell which is led by ethanol is less.This experiment adhibit TUNEL coloration, and flowcytometry examination and means of RT-PCR, as the different chroma and time ethanol pouring gorge rat's ovarian follicular and the different chroma and time ethanol bringing up granule cell as experimental datum, from cellular configuration, percentage of apoptosis and genie expression aspect and so on carry through examine. The aim is to explain toxic mechanism of alcohol, accordingly for aftertime coach of prepotency offers thereunder of defense and hygienics. The result of experiment said that :in the configuration we can see that along with the drinks dose temporal increase, phases of follicular bosom granule cell apoptosis degree distinctly boosts up; for different chroma and time,TUNEL coloration of granule cell induced by ethanol can be observed distinctly the morphologic character of cell apoptosis. The result of examine of flowcytometry showed that the apoptosis percentage of the granule cell induced by ethanol increased in a certain bound of chroma and time as the increasing of the chroma and time, and second diploid hump was observed obviously; the cell proportion of G0/G1 phase in ethanol group is more than antitheses group, and the cell proportion of S,G2/M phase in antitheses group is more than ethanol group , that is to say high chroma ethanol lead to cytoclasis; FasmRNA, FasLmRNA in granule cell all have expression, and as the increasing of the chroma and time it has the increasing dendency; but expression of the Bcl-2mRNA is weaken as the increasing of the chroma and time; Bax gene has expression also , but don't havedistinctlychange.lt shows that Fas, Fast, Bcl-2 gene in granule cell apoptosis process have action by all means. The result shows that ethanol can induce the apoptosis of granule cell.The damage action for ovarian awakes people that drinking show by abstention , and a stitch in time save nine which have a high flesh and blood meaning for human's procreate and health.