| Purpose: to develop HCT-8/5-FU multidrug resistant cell line and elucidate the effect of Chuan-hu-ning (an extract essence from medicinal herb) on HC1-8/5-FU multidrug resistant cell line.Method: HCT-8 cell line was used and high concentration of 5-FU was introduced at beginning to induce drug resistance, then the of concentration 5-FU was increased in gradient. About 7 month later, the cells can stably grow in 2.0 ug/ ml 5-FU, which was named HCT-8/5'-/ |
PDF Full Text Request