1.Mutational Analysis Of The Genes Susceptible Or Resistant To HIV-1 Infection Among Intravenous Drug Users And Patients With Sexually-transmitted Diseases In China 2.Foundation And Identification Of Monoclonal Antibody For D Peptide Of HBV Subtype

To evaluate the susceptibility at high-risk populations to HIV-1 infection, we analysed mutations in the chemokine coreceptor such as CCR5, CCR2, CXCR4, CX3CR1 and mannose-binding protein (MBP) allele frequency among a group of Chinese intravenous drug users (IDUs) and a goup of pepople with sexually-transmitted disease (STD).Blood samples were obtained from 210 Xinjiang IDUs and 189 STD donors in Henan Province. Genomic DNA samples were purified from the whole peripheral blood of subjects. Gene frequency was identified by means of PCR or PCR-RFLP. The significance of the data was analyzed by χ2 testThe frequencies of the CCR5-Δ32,CCR2b-64I and SDF1-3'A were as follows:2.14%,18.81% and 22.86%,respectively.Distribution of the three mutant alleles among the IDUs was in consistent with Hardy-Weinberg equilibrium. Statistical analysis showed that there were higher frequencies of CCR5-Δ32 and CCR2b-64I in HIV-1-positive IDUs than in those negative ones. No statistical difference in CCR5-Δ32,CCR2b-64I and SDF1-3'A was found between male and female individuals. It was found that the appearance of CCR5-Δ32 alleles influenced the gene frequency of CCR2b-64I.In individuals with the genotype of CCR5, the genotype of CCR2 is in distribution of Hardy-Weinberg equilibrium.However,in individuals with CCR5-Δ32 heterozygote,the frequency of CCR2b-64I decreased from 19.29% of wild-type CCR5 to 11.11% of heterozygote CCR5 and there was noCCR2b-64I homozygous. In CCR2b-64I, the gene frequency of CCR5-Δ32 also decreased from 2.65% of wild-type CCR2b to 1.43% of heterozygote CCR2b.There was no CCR5-Δ32 heterozygote in CCR2b-64I homozygous.The gene frequency of CX3CR1-249 was 16.67% in Xinjiang IDUs but 1.72% in Han ethnic origin. Meanwhile, the distribution of mutational alleles frequency was in consistent with Hardy-Weinberg equilibrium. The gene frequency of CX3CR1-249 was 23.33% in HIV-1-negative Xinjiang IDUs and 12.32% in those positive ones (P<0.05). The frequency was 4.73% in HIV-1-positive Henan STD Han people.The gene frequency of MBP-54 was 13.96% in Xinjiang IDUs and 14.91% in Han people. The distribution of mutational alleles frequency was in consistent with Hardy-Weinberg equilibrium. The gene frequency of MBP-54 was 13.04% in HIV-1-negative Xinjiang IDUs and 15.48% in those positive ones (P>0.75). The frequency was 16.25% in HIV-1-negative Henan STD Han people but 15.44% in those positive ones (P>0.5).MBP-52 and MBP-57 were not detected.The correlative analysis between SDF1-3'A and MBP-54 in Xinjiang IDUs showed that the p value was over 0.05. However, it was also found that the appearance of SDF1-3'A alleles influenced the gene frequency of MBP-54.Conclusion:1. We have detected the gene polymorphisms of CCR5-Δ32,CCR2b-64I,SDF1-3'A and CX3CR1-249,MBP-52,MBP-54,MBP-57 in IDUs and STD pations.2. The distribution of five mutational alleles frequency was in consistent with Hardy-Weinberg equilibrium.3. It was found that the appearance of CCR5-Δ32 alleles influenced the gene frequency of CCR2b-64I.The allele CX3CR1-249 mutation was associated with the genetic4. susceptibility to HIV-1 infection and accelerated disease progression possibly.5. The gene frequency of CX3CR1-249 is higher in Uigur ethnic origin than in Han IDUs.6. No Statistical difference of the allele frequency of MBP-54 was found among high-risk populations and healthy people.7. The mutational frequency of MBP-52﹑MBP-57 in Chinese would be extremely low.8. It was also found that the appearance of SDF1-3'A alleles influenced the gene frequency of MBP-54. 9. The mutational alleles frequency in high-risk populations and local healthy people was similar, which suggested that high-risk action has no effct on the allele frequency of associated with HIV-1 infection.10. The effect of those alleles on HIV-1 infection and disease progression among IDUs and STD pations remains to be clarified by long-term follow-up.