Study Of Baoganning On Treatment Of Liver Fibrosis

Objective:To study the effects and the mechanism of Baoganning on liver fibrosis.Methods:The experiment was composed of two parts,in vitro and in vivo. In vivo,the classical animal model of CCU was produced;two series of items were used to observe the effects,the degree of liver damaged and the degree of liver fibrosis. In vitro,the hepatocytes were separated by the method of in situ perfusion enzyme digestion,and damaged by Hydrogen peroxide or carbon tetracholride,the levels of ALI\ SOD> MDA> NO etc in the supernatant were detected. To further understand the mechanism of Baoganning,the change of NF-icB in liver cells injured by Hydrogen peroxide was researched,the effects on proliferation of hepatic stellate cell and secreation of type I collagen were observed too.Results:In vivo,the levels of ALT> MDA,HA> LN in serum could be decreased by Baoganning,and the level of SOD were increased. The collagen in the liver tissue was showed being reduced in Masson dying. In vitro,the levels of ALT\ S( MDAN NO etc in supernatant were reduced by both Baoganning. Baoganning could restrain the proliferation of Hepatic stellate cells and the secretion of type I collagen.Conclusions:Baoganning could retain the progression of liver fibrosis. The mechanisms might be:1. the function of anti-oxidation,2. the function of lessening the inflammatory reaction in liver tissue,3. the function of protection of hepatocytes,4. the function of restraining the proliferation of Hepatic stellate cells,5. the function of lessening the secretion of type I collagen.