| Objective: Along with material civilization and spiritual civilization constantly raising, living standard of modern times constantly improved, the desire of people to the long healthy life is also more and more strong. Therefore, how to delay senescence, and achieve the purpose of the long healthy life, the subject has become now one of the most important ones of world medical science circle. At present, the research to senescence mechanism of modern medical science involves all directions, multi-level and many factors such as ecology environment, organism , organisation , cell , molecule and gene etc . There are particularly more researches in the free radical theory, immunity theory and nerve theory etc among them. The kidney emptiness inducing senescence theory is the senescence theory of tradition in the traditional Chinese medicine. This theory considers the kidney emptiness is the basic cause of senescence. Jingqi of kidney from vigorous to deficient is the inherent mechanism of leading to organism constitution structure and physiological function to fail gradually. The research progress to kidney substance of recent years indicated that the kidney of traditional Chinese medicinecan summarize the whole or part of physiological function and pathological changes of each system such as hypothalamus-hypophysis-target gland axle and nerve, endocrine, immunity and biochenistry metabolizeing etc. Along with senescence, the internal environment function of each hierarchy and each system is low and disorder. Therefore the kidney emptiness inducing senescence theory of traditional Chinese medicine is declining to concern to the free radical theory, immunity theory and the nerve theory etc occupying the important seat in the senescence theory. Obviously, it possesses the characteristic of wholeness and comprehensiveness. A large number of experiments and clinical researches indicate that brain function descends at most of old age kidney emptiness persons, and the cerebrum nerve cells reduce, transmitter content and transmitter receptor quantity all descend. The immunity function descends. Immunity to oneself and allergy increase. The content of free radical and its peroxide increase along with age, but the content of matter of anti-free radical injury such as SOD etc descends. In view of this, according to the kidney emptiness inducing senescence theory we establish the Wenshen Tianjing Rule, and representative prescription Youguiwan adding other drug are chosen on this ground rule, that act on D-galactose senescence model of rat, and test the weight of thymus gland and spleen, the learning and memory capability of animal, interleukin-2(IL-2) of the blood serum, superoxidedismutase(SOD) and monoamine oxidase(MAO) in the brain tissue, malondialdehyde(MDA) in the liver tissue etc chang of markers. From many angles such as nerve, immunity and free radical etc inquire into " the Wenshen Tianjing Rule " delays senescence inherent mechanism, and provid theory and experiment basis for clinical medication.Methods: Improve a little according to the document, and take SD male rats of 48, that are randomly divided into 4 groups in adaptability feeding one week, namely the contrast group, the model group, the big dosage group and the small dosage group, per group 12. D-galactose is dissolved into brine to make the thickness be 5%, then by 0.1ml/100g given rat subcutaneous injection behind the neck for the model group rat, the big dosage group, the small dosage group, one time everyday, used continuously six weeks. Senescence mode is builded. The saline of equal dosage is given subcutaneous injection behind the contrast group rat neck. At the same time, the small dosage group according to 11.52g/kg is perfused the traditional Chinese medicine. The big dosage group is small dosage group of 2 times. The contrast group and the model group are perfused equal volume pure water. The rat jumping stand experiment is tested in 6 weeks, and test the change of rat praxiology , double test after 24 hours . After 24 hou...
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