| Obejective: Obesity is a risk factor of type 2 diabetes mellitus.It occurrence attributes to energy metabolism disequilibrium. By the traditional knowledge, obesity patient existed insulin resistance in vivo, and it's manifestation style is hyperinsulinaema. But with the improvement of insulin measurement, it was found that hyperinsulinaema in the patient of insulin resistance contained lots of proinsulin, and the proinsulin has little biologic activity. The insulin that has biological activity is called "true insulin". However, the "true insulin" has been measured little from now on. In addition, when obesity, the pancrease as a organ of secreting insulin, it's structural change is lack of morphology evidence.Method: 40 healthy Sprague Dawlay male rats (21 days initial age, weaning) were used for two groups, 20 in treated group and 20 in control group. They were assigned to the following treatment for 10 weeks: the treated group was fed high-fat food and the control group was fed normal food. At the same time of every weekend measured body weight, body length and LEEindex. 10 weeks later, choose the obesity and normal rats, were killed. Blood sample drawn from inferior vena cava was used for the determination of blood glucose, insulin and C-peptide. Then take out of the pancrease immediately, and fixed, gradient alcohol desiccation, embedded, section and ultrasection with light and transmission electron microscope (LM/TEM).Result: 1 The rat body weight change: From the third week, the body weight of rats in treated group increased significantly. From the fifth week, the body weight of rats in treated group increased significantly very much, and it lasted to the tenth week. Among them the seven week is extraordinary significance. When the end of this experiment, produced 14 fatty rats. 2 The index of blood glucose and insulin2.1 The blood glucose and insulin in treated group were higher than that of control group, and showed significant difference. The C-peptide in treated group has no difference with control group.2.2 The insulin sensibitity of treated group is short of control group.3 The normal rat's pancreatic tissue is chiefly compared of glandular lobules formed with acinus and connective tissueseparating these lobules. Pancreatic ducts, blood and lymphatic vessels, myelinated and unmyelinated nerve fibers, passed within connective tissue. The fatty rat's pancreatic tissue is of part of acini cell and islet cell fatty degeneration and atrophy. The islet is of antrum structure and plasm erythrodye, nucleus structure is unclear. The connective tissue fiber hyperplasia in acinus, lymphocytic cell infiltration, and seem to form lymphatic follicle. There was little fibroblasts proliferation.Conclusion: 1 After fatty-food induced rats for 10 weeks, the fatty rat reproduction rate is high, and it shows that the method bases on this experiment is reasonable.2 The blood glucose and insulin are higher than that of normal rats, but the change of C-peptide is not significant. It shows that the fatty rat are the earlier period of insulin resistance. 3 The structural feature of pancreatic tissue of fatty rats shows that long-term eating fatty food induces the material metabolism in vivo, and results in the morphology change of fat infiltration, inflammatory cell infiltration, the acinus cell and islet cell atrophy, connective tissue fiber hyperplasia.
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