| Objective : Studying the regularity of VEGF-C and COX-2 expression in Hepatocellular Cancer(HCC) , paracarcinoma tissues and normal liver tissues. HCC is one of the highest incidence malignant tumors in our country which is easy to metastasis in the early stage and has a high rate of recurrence after operation. In the present, the development of preventing recurrence and metastasis still plays a pivotal role in clinic. Angiogenesis,which was found in recent years, has important relationship in the process of tumor formation and development, and neoplasm metastasis and neoplasm invasiveness has important relationship in the process of lymph node hyperplasia. Vascular endothelial growth factor C (VEGF-C) discovered recently is a new member of Vascular endothelial growth factor (VEGF) family. Studies have shown that VEGF-C is a specific regulating factor for lymphatic endothelial cells, and it expresses in a variety of human tumors. VEGF-C can accelerate the process of lymph node hyperplasia and lymph node metastasis, further more, it also has a function of accelerateing angiogenesis of tumor. VEGF-C and COX-2 are both the factors that accelerate the tumors growth. There is close relationship between VEGF-C and COX-2 in tumor's formation and development. Liver is rich in blood vessel. In the past, most of people thought that the vascular hyperplasia was the main factor in recurrence and metastasis of HCC, but they all neglected the function of lymph system In this study, we detected the expression of VEGF-C and COX-2 in HCC tissues, paracarcinoma tissues and normal liver tissues to clarify the role of VEGF-C and COX-2 in the invasion and metastasis of HCC, as well as in invasion of vascular and lymphatic, in order to explore new approaches of preventing recurrence of HCC. Methods: VEGF-C and COX-2 were detected in 43 HCC specimens , paracarcinoma specimens and 24 case normal liver tissues specimens by immunohistochemical technique. VEGF-C and COX-2 were analyzed difference of expression in three cases tissues with methods of statistics, so were the relationship between expression of VEGF-C and COX-2 both and the clinic pathologic character,as well as the relationship between VEGF-C and COX-2.Results: 1 The expression of VEGF-C: There were 31 cases high expression of VEGF-C, 12 cases low expression and no-expression in carcinoma tissues. However, there were 10 cases high expression of VEGF-C, 33 cases low expression and no-expression in the paracarcinoma tissues.3 case showed high expression of VEGF-C, 21 cases showed low expression and no-expression in 24 case normal liver tissues.The expression level of VEGF-C in the carcinoma tissues was significantlyhigher than that in paracarcinoma tissues and the normal liver tissues.These results showed that the expression of VEGF-C enhanced in HCC,and there were statistic difference between the carcinoma tissues and paracarcinoma tissues or the liver tissues(P<0.05). The expression level of VEGF-C in paracarcinoma tissues was higher than normal liver tissues, but there were not statistic difference between them.2 The expression of COX-2: There were 28 case high expression of COX-2, 12 cases low expression and no-expression in carcinoma tissues. However, there were 26 cases high expression of COX-2, 17 cases low expression and no-expression in the paracarcinoma tissues,There were 3 case high expression of COX-2,21 cases low expression and no-expression of COX-2 in 24 case the normal liver tissues.The expression level of COX-2 in the carcinoma tissues and the praacarcinoma tissues were significantly higher than that in the normal liver tissues, but there were not statistic difference between the carcinoma tissues and paracarcinoma tissues (P>0.05) These results showed that the expression of COX-2 enhanced in HCC and paracarcinoma tissues. There were statistic difference between carcinoma tissues or paracarcinoma tissues and normal liver tissues(P<0.05).3 The relationship between VEGF-C and the clinic pathologic character: the expression of VEG...
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