| PrefaceIn the early studies, Dean and his colleagues found the relation of fluoride and dental caries, and were sure of the caries - preventive effects of fluoride. Recently, much researchers consider that fluoride varnish can enhance the anticaries effect of dental enamel. Fluoride varnish films on the surface of dental enamel after topical application of fluoride varnish. F - ions release from the film, and then protect dental enamel. Now, topical application of fluoride varnish inaugurate new approach for the prevention of dental caries.In the present study, we i nvestigate the fluoride concentrations in whole saliva after topical application of fluoride varnish in vivo before all other researchers in China. At same time, with the analysis of the dissoved Ca from enamel after topical application of fluoride varnish , we assess effect on anti - acid solubility of fluoride varnish with dental enamel, in order to provide science foundation for clinical practice.Material1. 2% NaF varnish and 2% NaF solution2. Primary reagent2.1 Lan liquid2.2 TISAB liquid3. Primary instrument3.1 Centrifuge :Sorvall super T21, DUPONT Inc. ,USA 3. 2 Fluoride - sensitive electrode: Changsha semiconductor Inc. , China3.3 pH;PHS-3 C, Shanghai leici instrument Inc. , CHIN A3.4 Atomic absorption spectrometry: AA - 670, Shimadzu, JapanMethods1. Subjects20 caries - free students (10 Boys and 10 girls ) ranged in age from 15 to 17 years were selected and divided into NaF varnish and NaF solution groups.2. Analysis of fluoride concentration in salivaThe samples of unstimulated whole saliva were collected at baseline before a single topical application of NaF varnish or NaF solution and 1, 3, 6, 12, 24, 36h after a single topical application of NaF varnish or NaF solution. The concentration of fluoride in saliva samples was determined with a fluoride - sensitive electrode.3. Analysis of dissoved Ca from enamelThe samples of dissoved Ca from enamel were collected at before a single topical application of NaF varnish or NaF solution and 1,2,3 month after a single topical application of NaF varnish or NaF solution. The dissoved Ca from enamel was analysed with atomic absorption sectrophotometry( A A -670 Japan).4. Statistical methodsThe data were processed using the t test by SPSS7.Results1. Compared with baseline value and the values of salivary fluoride concentrations after topical application of fluoride varnish, the values after Ih, 3h were significantly higher than baseline( P <0. 01). Compared between fluoride varnish group and fluoride solution group, the values of fluoride varnish g;roup were significantly higher than fluoride solution group after lh(P<0.01); the values of fluoride varnish group were higher than fluoride solution group after 3h(P<0.05).2. Compared with baseline value and the values of dissoved Ca from enamel after topical application of fluoride varnish, the values after 1, 2, 3 months were significantly lower than baseline( P <0.01). Compared with baseline value and the values of dissoved Ca from enamel after topical application of fluoride solution, there were no significant differences between the values after 3 months and baseline ( P > 0.05 ). Compared between fluoride varnish group and fluoride solution group, the values of fluoride varnish group were lower than fluoride solution group after 1 month(P<0.05) ; were lower than fluoride solution group after 2, 3 months( P <0.01).Discussion1. According to the majority of studies, comparing with other topical fluoride products such as dentifrices or mouthrinses, fluoride varnish can prolong the time of its contact and reaction with enamel. Castioni et al. analysed the data of the concentrations of fluoride found in saliva after a single topical application have been investigated in many studies, and reasoned out that professionally applied products, containing high fluoride concentrations, lead to higher salivary levels as compared to self - applied p...
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