The Expression And Biological Significances Of Signal Transducers And Activator Of Transcriptions1, 2, 4 In Primary Hepatocarcinoma

Background: Hepatocarcinoma is a multicause,multistep and complex process. Recent research in molecular biology has shown the causes leading to primary hepatocarcinoma (PHC). Increasing evidence exists that hepatocellular transformation has two sections. Mutantion of gene and gene function regulation and control were abnormal. Signal Transducer and Activator of Trancription (STAT) control the expression of DNA caused over-priliferation of genes, which might play a important role in the tumorgenesis and predict the prognosis of patients with primary hepatocarcinoma.Objective: To investigate activation of STAT1, STAT2, STAT4 and their clinicalpath-ological significance in the central hepatocarcinoma tissues, Preicarcinrous liver tissues and normal liver tissues.Method: The resected specimens originated from 37 patients with PHC and 17 normal liver tissues(indentified by pathology). 37 specimans were divided into 37 central-cancer tissues and 37 pericancerous liver tissues. All these specimens were fixed in 10 percent formalin and routinely embedded in the paraffin. Then the specimens were continuously sliced into 4 u m thick sections. TheseSections were stained by streptoavidin-biotion method of immuohistory chemistry with polyclonal antibodies against STAT1, STAT2 and STAT4 protein. The data of positive expression and clinicalpathological characteristics was analysed by SPSS10.0 software.Result: Clinical assays showed that the positive rate and score of STAT1, STAT2, STAT4 was significantly lower in the central cancer tissues man that in the pericancerous tissues and normal liver tissues(p<0.05). The score of expression in well-differentiation stages was higher than that in poor-differentiation stages (p<0.05). The score of STAT4 was significantly higher in hepatocelleclar carcinomas than that in mixed hepatocarcinoma. It was clearly seen the score of protein's activator did not correlate with the other clinicpathological features of primary hepatocarcinomas (AFP, cirrhosis, metastasis, and maxial diameter of mass, respectively).Conclusion: (l)The expression activatory of STAT1, STAT2, STAT4 might be related to the formation of primary hepatocarcinomas. (2) Difference of differentitated degree indicated the reverse expression in primary hepatocarcinomas, which might help in predicting their prognosis.