| Nephroblastoma is one of the most common abdominal malignant tumor in children. It consists of 8% in all pediatric solid tumors. Max wilms descripted its characteristics in 1899, so it was named after his name. Nephroblastoma generates from metanephric blastemic rest cells, probably resulting from delayed and residual differntation of metanephric blastema. Its main prognosis factors are the histological structure, age, the totally excision of original tumor, metastasis and two sides tumor.In recent year, researchs towards tumor angiogenesis have become new hot spot, angiogenesis is regarded necessary for growth and metastasis in all solid tumors. Vascular endothelial growth factor (VEGF) is the most typical angiogenesis stimulator, overexpressing in many tumors. Matrix metallproteinase-9 can degrade matrixes, which contributes to neovascularization and dendritic extension of blood vessel. CD34 is a sensitive marker ofvascular endothelial cells, it can be used to detect tumor microvessel density (MVD), Which is widely used to evaluate the angiogenesis of tumor. To date, the angiogenesis activity of nephroblastoma and its regulators have not been well defined. In this study, angiogenesis in nephroblastoma, as well as the correlations among VEGF MMP-9 and MVD were analysised. The study aimed at evaluating angiogenesis and its regulating mechanisms in nephroblastoma, so as to provide a theoretical foundation for potential new therapy method (anti-angio genesis therapy) of nephroblastoma. Immunohistochemical streptavidin peroxidase method were performed in 41 samples of nephroblastoma, which were obtained from 41 patients who underwent surgery, to investigate the relationship among VEGK MMP-9 MVD and nephroblastoma.Materials and methods: we have collected 41 children nephroblastoma specimens (tumor group) and 12 normal renal tissue near the tumor (control group) from surgical resections, and all the sections were formalin-fixed and paraffin-embedded. The patients, from 4 months to 13 years, average 3.66, 27 males and 14 famales, were not performed any radiotherapy and chemotherapy. Their HE staining sections were affirmed and histopathologic types were classfied by two pathologist ,their clinical stage were classfied according to the conditions during operation and the pathological diagnoses after operation (by NWTS-3 criteria). Among them 6 patients had stage I tumor, 20 stage II, 14 stage III, 1 stage IV; 33patients had FH (favorable histology) type tumor, 8 UH (unfavorable histology) type. VEGF, MMP-9, CD34 expression on 41 tumors, 12 renal sections were detected by immunohisto-chemistry streptavidin peroxides methods. The results are analysed by SPSS 10.0 statistical software, a equals 0.05 were considered significant test level.Results: (1) The positive rates of VEGF expression in nephroblastoma and control groups were 58.54% (24/41), 25.00% (3/12) respectively, there was significant difference (P<0.05). (2) The positive rates of MMP-9 expression in nephroblastoma and control group were 63.41% (26/41), 16.67%(2/12) respectively, there was significant difference (P<0.05). (3) There were no obvious relationships between VEGF, MMP-9 and sex, age groups (P>0.05). (4) In FH type tumor group and UH type tumor group, the positive rate of VEGF expression were 54.55% and 75.00% respectively, no significant difference was found (P>0.05); the positive rate of MMP-9 expression were 57.58% and 87.50%, no significant difference was found (P>0.05). (5) The positive rates of VEGF expression in clinical stage I+II and clinical stage III+IV were 42.31% and 86.67% respectively, it has significant difference between them (P<0.05). The positive rate of MMP-9 expression in clinical stage I+II and clinical stage III+IV were 50.00% and 86.67% respectively, it has significant difference (P<0.05). (6) The MVD of nephroblastoma was 37.72+17.36, the MVD of controlgroup was 31.36+5.11, there was significant difference between them (P<0.05). MVD was not related to sex and age group (P>0.05). In FH type tumor gr... |
