Effects Of OX-LDL On Cell Proliferation In Cultured Coronary Arterial Smooth Muscle Cells

Objective Atherosclerosis in the coronary and other arteries is characterized by a dysfunction of endothelium,chronic inflammationjipid accumulation;growth of smooth muscle cells (SMC). Intimal SMC proliferationand and collagen production are important for the evolution of a mature and obstructive coronary plaque.Oxidized low density lipoprotein(OX-LDL) plays important roles in the formation of atherosclerotic lesions.The purpose of the present study isto better understand the effects of oxidized low density lipoprotein (ox-ldl) on cultured coronary smooth muscle cells proliferation.Methods 1 cell line cells came from swine coronary SMC primary culture.Passages 2-3 SMC were used. 2 Low density lipoprotein were isolated from healthy human plasma by sequential ultracentrifugation and oxidized by CuSOj.Coronary SMC were exposed to nature-LDL,OX-LDL and lipoprotein-deficient serum( LPDS )as control .The ability of proliferation of cells were by MTT assay and the flowcytometric method in the present study .Results The results showed suitable concentration OX-LDL may allow SMC from contratile phenotype into synthetic phenotype.The ability of proliferation of the coronary SMC is increased when esposed to theOX-LDL.The percentage of s phase cells in the OX-LDL group was higherthan that of the control group .There is a positive relation between the OX-LDL mediated SMC proliferation and the cycle phase shifting.Cone I us i on The ability of proliferation of the coronary SMC is increased when esposed to the OX-LDL.The OX-LDLmediated coronary arterial SMC proliferation is related to increasing cells during S phase .