Experimental Study On The Theraputic Effect Of TP On Lewis Lung Cancer

Objective: To observe the inhibitory effect of Tea Polyphenol(TP) on tumor growth, anti-oxidation and immunoregulation, and to investigate its effect on apoptosis of tumor cells in order to clarfy its multi-targeted anti-tumor effect. Method: Tea Polyphenol was administered by gastric lavage to C57BL/6J mouse implanted with Lewis lung cancer for 12 days in doses of 62.5mg/kg,125mg/kg and 250mg/kg respectively. Inhibition on tumor growth, antioxidation, and immunoregulation were observed and its effect on apoptosis of lung cancer cells was investigated at the same time.Results: Tea Polyphenol at dose of 62.5mg/kg showed no inhibition on Lewis lung cancer cells, inhibitory rates of TP at doses of 125mg/kg and 250mg/kg were 27.2% and 18.8% respectively, with significance over controls at dose of 125mg/kg. Although no effect on spleen in normal mouse at dose of 125mg/kg was found, thymus weight was reduced significantly and atrophy of thymus of tumor-bearing mouse was augmented further. Spleen index in tumor-bear mouse was reduced significantly at dose of 125mg/kg and 250mg/kg. Serum GSH-Px activity was increased significantly and serum MDA levels decreased in all 3 groups. SOD activity in 125mg/kg group was increased more significantly than those in controls. Apoptosis was found in both groups at doses of 125mg/kg and 250mg/kg.Conclusion: Tea polyphenol can inhibit the growth of lung cancer, the underlying mechanism of which was associated with antioxidation and induction of apoptosis.