Experiment Study Of Intra-Radiotherapy With ～(32)P-Glass Microsphere On Hypersplenism

Objective: l2P-Glass Microsphere (32P-GM) was used as material in spleen artery embolization, study the efficacy and safety of ""P-GM on experimental hypersplensim, to find out a new way with better effect and little side effect to treat hypersplenism. Methods. 32P-Glass Microsphere was used as embolizing material, firstly: experiment of dosage selection with rat. 25 experimental cirrhotic rat divided into 5 groups randomizely, treated with 0 30 60 120 240 uCi/g (spleen) of 32P-Glass Microsphere respectively by intrasplenic injection directly. Blood routine test was performed. Pathology of spleen, liver, kidney, lung and heart was checked to screen the fittest effective dosage. Secondly: treatment of experimental hypersplenism dog. 5 of experimental hypersplenism model dog obtained by ligation of spleen vein directly were treated with 60~120 uCi/g (spleen) of "2P-Glass Microsphere. Blood routine and pathologic examination of spleen, liver, kidney, lung and heart were checked to prove the efficacy and safety of this dosage of l2P-Glass Microsphere. Result: White bloodcell count of rats increased at the first week after intrasplenic injection. Blood platelet count elevated a little late to significant level at about the fourth week, both got to peak at about 4-8 week, and maintained at significant high level more than 12 weeks. Hemoglobin, red blood cell, alanine transaminase(AJLT), blood urine nitrogen(BUN) and creatinine(Cr) didn't change significantly. Pathologic examination showed spleen crimpled obviously, splenic corpuscles shrank and surrounded with increased fiber tissue in groups more than 60 uCi/g. 240 uCi/g group showed spot congestion of capillary and inflammatory cellular infiltration in lung. The white blood cell and platelet count of 5 hypersplesism dog treated with 60-120 u Ci/g(spleen) of ~l2P-Glass Microsphere elevated at the first week post treatment which peaked at 4 weeks and decreased a little but still maintained at high level more than 3 months. Pathology showed the size of spleen reduced 32. 78% ~ 65. 32% with obvious fibrosis. Conclusion: Rat experiment showed treating with 60-120uCi/g(spleen) of ' P-Glass Microsphere can either cause significant increase of white cell and platelet count or induce spleen fibrosis withlittle adverse effect. Therapeutic experiment of hypersplenism dogproved the efficacy and safety of 32P-Glass Microsphere embolization.