| Objective: To investigate the effects of 1,25-dihydroxyvitamin D3 on the bone metabolism, bone capacity and primary osteoporotic fracture healing in order to provide theoretical basis of searching better auxiliary therapy for primary osteoporotic fracture patients.Methods: 20 8-month-old female New Zealand white rabbits were randomized into two groups: ovariectomized (experiment group, 15 rabbits) and pseudo-ovariectomized (Sham group, 5 rabbits). Five rabbits each of the two groups were determined biochemical indexs and bone mineral density(BMD) for the confirmation of successful establishment models of postmenopausal osteoporosis in 3 months postoperatively. The 15 animals of experimental group were re-divided into 3 groups averagely: ovariectomized group(OVX), calcii gluconas group(OC), calcii gluconas and 1,25-dihydroxyvitamin D3 group(OCR). The BMD of lumbarvertebraes and femurs of five rabbits each of 15 3-year-old female , 15 4-year-old male , 5 1-year-old female and 5 1-year-old male New Zealand white rabbits were determined. 15 rabbits each of the 3-year-old female and the 4-year-old male rabbits were acted as models of senile osteoporosis and were randomized into 3 groups averagely: control group(Control), calcii gluconas group(Calcium), calcii gluconas and 1,25-dihydroxyvitamin D3 group(CR). Then all of experimental animals were made as models of both sides fractures of radius.In next day, OC group and Calcium group were given calcii gluconas 0.5g/(kg.d), OCR group and CR group were given calcii gluconas 0.5g/(kg.d) and 1,25-dihydroxyvitamin D3 0.025 u g/(kg.d). The time was lasted 8 weeks after the animals were given drug. All the experimental animals were determined biochemical indexes in 4,8 weeks postoperatively and shot the X-grams of fractures in 2,4,8 weeks postoperatively . The BMD of the bony callus and lumbar vertebraes of all experimental animals were determined in 8 weeks postoperatively. Then all the fracture specimens were taked from the experimental animals and the bony callus were observed histologically and determined bone tissue morphometry.Results: Serum bone Gla-containing protein (BGP) and urinary calcium/creatinine (Ca/Cr) were increased but the BMD of lumbar vertebraes and femurs were decreased inovariectomized group compared with Sham group (P<0.05 or P<0.01) in 3 months after ovariectomizing operations. The BM D of lumbar vertebraes and femurs were decreased in senile female and male rabbits compared with 1-year-old female and male rabbits respectively (P<0.05 or P<0.01). It was 8 weeks after all the experimental rabbits were given drug. All serum calcium, serum phosphorum, alkaline phosphatase (ALP), X-ray score, the BMD of lumbar vertebraes and femurs and the average of bone volume and trabecular plate thickness among bone tissue morphometry were increased significantly in OCR group compared with OVX group and OC group (P<0.05 or P<0.01). All BGP, urinary Ca/Cr and trabecular spacing were decreased significantly in OCR group compared with OVX group and OC group (P<0.05 or P<0.01). All serum calcium, BGP, ALP, X-ray score, the BMD of lumbar vertebraes and femurs and the average of bone volume and trabecular plate thickness were increased significantly in female and male CR group compared with Control group and Calcium group (P<0.05 or P<0.01). Urinary Ca/Cr and trabecular spacing were decreased significantly in CR group compared with two other groups (P<0.05 or P<0.01). Not only all of determined indexes were not difference significantly in OVX group compared with OC group but also in Control group compared with Calcium group (P>0.05). All of determined indexes were not differencesignificantly in Sham group compared with OCR group (P>0.05) but serum calcium, serum phosphorum and ALP.Conclusion: (D The models of postmenopausal osteoporosis and senile osteoporosis were established successfully. (D 1,25-dihydroxyvitamin D3 can enhance the concentration of serum calcium and serum phosphorum to postmenopausal osteoporosis and the concentration of serum calc...
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