Study Of Radiosensitizing Effect Of Paclitaxel On Human Adenoid Cystic Carcinoma Acc-2 Line Cell In Vitro

Object: To investigate the radiosensitizing effect of paclitaxel on human adenoid cystic carcinoma Acc-2 line cell and its primary mechanism in vitro. Methods: we calculated the survival percent of human adenoid cystic carcinoma Acc-2 line cells treated by paclitaxel and (or) radiation at different time and with different concentration by cell cloning Assay, and then sensitizer enhancement ratios (SER) were evaluated. Paclitaxel-induced arrest of cell mitosis and apoptosis was detected with flow cytometer. Results: Paclitaxel inhibited Acc-2 proliferation in vitro. Pretreated with 10, 100, lOOOnmol paclitaxel for 24h, the SERs were 1.29, 1.66, 1.23 respectively. The cells developed a G2/M arrest after exposured to paclitaxel. The percentage of the cell population in Gi/M was higher at longer paclitaxel exposure time and at higher paclitaxel concentration. Pretreated with 100, lOOOnmol paclitaxel for 24h, the percentage of the cell population in Gi/M was increased significantly. The percentage of the cell apoptosis was 20.7% , pretreated with lOnmol paclitaxel for 24h. Conclusion: Paclitaxel has radiosensitizing effect on human adenoid cystic carcinoma Acc-2 line cell in certain condition in vitro. The radiosensitizing effect of paclitaxel maybe a multi-mechanism event. Paclitaxel-induced Gz/M arrest and apoptosis play an important role in it.