Protection Effects Of Titanoreine On Stress Ulcer In Rats: An Experimental Study

Background: Although the incidence of bleeding from stress ulcer has decreased markedly, there is no effective therapys can prevent it if stress ulcer really happened. Titanoreine suppository, a protector of mucosa, has been demonstrated clinical effective in the prevention of hemorrhoids. My study is to explore Whether it is effective in the prophylaxis for stress ulcer too.Objective: to investigate the protective effects of titanoreine on stress ulcer. Methods: Thirty male wistar rats, each weighing about 200g, were randomly assigned to group as follows: 1) NS control (5ml/kg), 2)escipient (5ml/kg), 3)low-dose titanoreine (0.15g/kg), 4)high-dose titanoreine(0.75g/kg), 5)cimetidine (0.5g/kg) treatment group. All drugs were administrated intragastricaHy once a day for 4 days. After the last administration, All rats were fasted for 24h but were given free access to water. To avoid any diurnal effect, all animals were studied at the same time of day. The animals were placed in restraining devices and maintained at 23 ?.5癈 for 20h after general anesthesia induced by ether. At the conclusion of the experiment, rats wer sacrificed by cervical dislocation under light ether anesthesia and a middle laparotomy was performed. After ligation of pylorica, The stomach was inflated with 8ml of 1% formalin through esophagus. The total stomach was removed quickly and soaked in 2% formalin for 30 min after the cardia was also ligated. Then the stomach was open along the greater curvature and the longer diameter of ulcer was measured using vernier caliper under the magnifying mirror(X 10). Finally, each stomach was refixed in 4% formalin, and Paraffin sections were stained with hematoxylin-eosin for histologic observation.Results: stress lead to multiple gastric ulcerations in all animals. However, except the escipient group, the frequency, number, and size of gastric ulcers were significantly reduced in both titanoreine-pretreated and cimetidine-pretreated animals, especially in the high-dose Titanoreine animals. The ulcer index in the escipient group is not different from the control group(34.22?.24 vs 36.49?.49 P>0.05). The pretreamentgroups with low-dose Titanoreine, high-dose Titanoreine, and Cimetidine have a lower ulcer index, 14.03+2.03, 5.05 + 1.33 and 14.87+1.63 respectively, significant (p<0.01) different from the control group(36.49?.49). Among the three pretreatment groups, there is no difference between the low-dose Titanoreine group and the Cimetidine group, but the ulcer index was remarkedly reduced in high-dose Titanoreine group compared with that in cimetidine group (5.05 + 1.33 vs 14.44+ 1.39, PO.01). Control and the escipient animals displayed a loss of superifcial epithlium and necrosis of the upper mucosa in the histologic specimens. In striking contrast, gastric specimens from the pretreated animals displayed minimal or no changes.Conclusion: The protective effect of Titanoreine is superior to Cimetidine in the prophylaxis for stress ulcer, and may be a prospective agent for the prevention of stress ulcer.