The Dynamic Research Of Nitric Oxide And Endothelin In The Cerebral Hemorrhage

Intracerebral hemorrhage is a human and serious paralysis, with the one of the important reasons that cause death, but intracerebral hemorrhage roughly occupy all apoplexy the inside of 12%, but have than ischemic apoplexy higher cause death rate with paralysis rate. For deeply understanding succeed brain hurt after intracerebral hemorrhage, then guide clinical provide the theories and foundation to apoplexy's of basis, influence forwards cerebral blood circulation and brain organize research become a hot point. Nitric oxide and Endothelin just is regulation factor of the partial blood circulation of the detection's organ in recent years. Endotheliocyte passes to release ET and contract the vessel with promote endotheliocyte to propagate, and release NO torelax smooth muscle of vessel with repress endotheliocyte propagating at the same time. In the normal circumstance, the ET is placed in the equilibrium with NO and maintain function of relaxation and contraction in cerebral vessel, the equilibrium in two afferent function materials of the brain blood vessel is broken, then cause the blood vessel contract and the brain cell hurt. The apoplexy paroxysm the breakage of the development and this kind of function is take placed relevant.This research will pass to measurement apoplexy animal model blood plasma inside NO and ET-1 contain. Proceed the dynamic observational blood plasma NO and ET-1 variety, study to both influence apoplexy succeed brain hurt and its both it's relativity. Material and method K Experiment animal and handleFrom Zhejiang Medical science section college experiment animal center provide to the adult and big and white rat 108, heavy 250~300g, bisexual and each half. Animal model creation adoption is from the artery blood infuse into white rat's caudal nucleus, and result in the apoplexy the inside of animal model, 0. 9% sodium chloride injection makes to wait to measure to checkagainst.2 Experiment cent setThe big and white rat weighs the empress respectively to with the serial number, in the SPSS(vlO. 0) take the number cent three great groups with the machine:(1) 25ul group: 36 (bleed group 18^ control group 18)(2) 50ul group: 36 (bleed group 18> control group 18)(3) lOOul group: 36 (bleed group 18N control group 18) SN The sample collectFrom the artery blood or 0.9% sodium chloride injection infuse into the big and white rat's caudal nucleus empress 4h> 12h. 24h behind, break the rat's head and take the vein blood 4ml 2ml infuse heparin anti coagulation's grass tube, another 2ml infuse contain 10% Disodium Edetate with the of Aprotinin inside. 4 NO and ET-1 measurementThe blood plasma NO contain measurement adopts nitric acid restore and ET-1 contain measurement adopts radioimmunity method.Experiment result 1 the blood plasma NO contain to measure at apoplexy express4h 12h and 24h show to descend the trend with match controlcompare to have to show significant difference and after apoplexy 24h NO contain is lowest.2 blood plasma the ET-1 contain to measure at apoplexy 4h> 12h and 24hs, all go up and compare with match control to have to show significant difference, after apoplexy 12hs is highest.3 Same descend to contain with ET-1 to measure to go up to show out the deal set blood plasma NO related.Conclusion1 the blood plasma NO contain to descend, the ET-1 to contain to measure to go up with experiment intracerebral hemorrhage during acute period.2 After the apoplexy the blood plasma NO contain to measure the descent and blood plasma the ET-1 to contain to measure to go up to show related contract, express the NO and ET-1 to be used as the partial blood circulation of an organ to down be placed in the dynamic equilibrium apoplexy to behind break this kind of equilibrium may cause succeed brain hurt.