P-glycoprotein Expression And Its Relationship With Mutated P53 In Gallbladder Carcinoma

Introduction: The tumor cell's drug resistance is an important reason for chemotherapy failure. The most important factor is the multidrug resistance phenomenon (MDR). The occurrence of MDR is associated with P-glycoprotein (P-gp) which is due to the over expression of MDR- I gene. The abnormal expression of p53 is involved in the tumor's occurrence and development. To explore the regulation linked mechanism of MDR, we investigated concomitant expression and correlation of Pgp and p53 in gallbladder's neoplasm, which was analyzed by irnmunohistochemical method. Materials and Methods: Gallbladder carcinoma tissues were obtained from 41 patients having surgery in JinHua Municipal Central Hospital and JinHua Municipal people's Hospital during 1995-2000. Serial section of gallbladder carcinomas were adopted for concomitant expressions of p53 and Pgp by streptavidin-peroxidase(SP) irnmunohistochemical staining while the expression in chronic cholecystitis were 6 adopted as controls. Results:The irnrnunohistochemical staining of p53 and Pgp in serial sections of human gallbladder carcinoma tissues have produced expected positive signals with clear background. The negative control shows no signal. The Pgp positive staining is located in cell plasma. The expression rate of Pgp in gallbladder carcinoma is 56.1 %, which is higher than the expression in chronic cholecystitis (p