The Epidemiologic Study On The Polymorphisms Of TAP Gene And Relative Factors Of DM

Objective The polymorphisms of transporter associated with antigen processing(TAP) genes were examined in 16 Chinese patients with type 1 diabetes mellitus and 50 health controls as subjects. Methods Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for discriminating two dimorphic sites of TAPI gene and four dimorphic sites of TAP2 gene. Amplified products from genomic DNA were digested with restriction endonucleases: Sau3Al for TAP1 codon 333 (Ile-Val), AccI for codon 637 (AspGly), and BfaI for codon 687 (Stop-GIn). The other site of TAP2 gene were analyzed by mismatch PCR-FRLP: Bstul for TAP2 codon 379 (Val-Ile), RsaI for codon 565 (Ala-Thr), and MspI for codon 665 (Thr-Ala). Results We have found in TAP 1 allele were most 637Gly in type I DM and mainly 637Asp in control group. In TAP2 allele, were most 3791le in type I DM and mainly 379Val in control group. We observed four TAPI alleles and seven TAP2 alleles in two groups. The alleles phenotype frequencies of TAP 1B, TAP1C and TAP2D were significant differences between two groups(P<0.0 1). Conclusions We identify any differences of TAP gene polymorphisms between two groups.