The Experimental And Clinical Studies Of Corneal Preservation By Organ Culture

Objective To observe the effects of cornea! preservation by organ culture and to disscuss the feasibility of this method. Methods 54 porcine corneas were divided into two groups, experimental group(30 corneas) and control group( 24 corneas). The corneoscleral discs of experimental group were preserve in 31 慍 organ culture media. Every 6 corneas were preserved for 3, 7, 14, 21 days respectively. 6 corneas were incubated in organ culture medium containing 5% daxtran 500 for three days after 14 days of organ culture preservation. Before preservation, corneas were checked for corneal thickness, morphology, staining of trypan blue and density of endothelium. At different time of preservation, the same check items and staining of alizarin red were made. The corneas of control group were preserved in 40C K-solution. Every 6 corneas were preserved for 3, 7, 14, 21 days respectively. The same obsrvations with experimental group were made. At different time of preservation In two groups, endothelium were studied by TEM in one cornea. The samples of media were removed from all bottles for bacterial and fungal culture 2 before opening bottle. 28 rabbit corneas were divided into two groups, 16 for experimental group and 12 for control group. In experimental group, every 4 corneas were preserved in 31 揅 organ culture media for 3,7,14 days respectively. 4 corneas were shifted to 31 揅 organ culture media with 5% daxtran 500 and kept for 3 days after 14 days preservation in organ culture. In control group every 4 corneas were preserved in 4擟 K-solution for 3, 7, 14 days respectively. Before preservation the thickness of corneas was measured and endothelium was evaluated for morphology and trypan blue staining. After presrvation, the same check items and staining of alizarin red were made.The sterility of media were checked in the same way with porcine cornea preservation media. Result In porcine corneas, after 7 days of preservation, there were obviously difference in morphology and viability of endothelium between two groups. The rate of viable endothelium of experimental group was higher than control group with statistically difference at 7, 14, 21 days after preservation. The endothelial density of experimental group decreased with prolongation of preserved time. The percentage of cell loss was higher in experimental group than in control group after 7 days of preservation with statistically difference. There were statistically differences in corneal thickness between two groups after 3 days of preservation. The thickness of corneas decrease obviously after 3 days preservation in 5% daxtran supplied organ culture medium and there was no obviously hamper on endothelium The results of TEM showed that the changes of endothelial ultrastructure between two groups were obviously different. The peservation of organ culture maintains endothelial integrity for longer time than control group. In rabbit corneas, there were many differences in morphology of endothelium between two groups. After 7 days preservation, the rate of viable endothelium of experimental group is statistically higher than control group at 7 and 14 days of preservation. There were statistically differences in corneal thickness between two 3 groups. The rate of microbial contamination was 10.4%,...