Establishment Of An Animal Model Of Carotid Stenosis And Studing Of Cartoid Endarterectom For Treating The Stenosis

Establishment of an Animal Model of Carotid Stenosis and Experimental Studing of Cartoid Endarterectomy for Treating the Stenosis Department of Neurological Surgery. Wuhan General Hospital of PLA Postgraduate: Zhang Xiaolun Supervisor: Ma Lianting, Wu Zuoquan Abstract Objective: Establishment of an Animal Model of Carotid Stenosis by endarterium hyperplasia caused by carotid endothelial cells injury; Assessing the degree of carotid stenosis by colourful duplex sonography (CDS) and selective carotid digital subtraction angiography(DSA) ;To determine the curative effect of carotid endarterectomy on carotid stenosis in rabbits. Methods and grouping: 1. The carotid endotbelial cells were injured by several ways: The carotid endothelial cells were injured only by mechanical method in group A Only by hemodynamical method in group B; By combination of mechanical and hemodynamical method in group C; Do not injury endothelial cell in control group D. CDS and slective DSA of the carotid artery were performed; Cholesterol were examined two weeks after the injury in all survival animals. The carotid arteries that become stenosal in two animals were taken for histological examination. 2. That the degree of carotid stenosis was greater than 50 percent (confirmed by CDS and DSA) in 14 rabbits, Who underwent carotid endarterectomy to eliminate the stenosis, CDS was performed immediately and one month, two months and three months following the operation. Results: -3- 1. There is no significant difference between the content of cholesterol in all experimental groups and the control group 2. Colourful Duplex Sonography: (1) The carotid stenosis induced by endoarterium hyperplasia and endovascular vegetation was revealed by two dimensonal imaging; (2) Color doppler imaging reveal the endovascular color blood stream was not smooth; (3) Frequency spectrum doppler imaging reveal the peak velocity increase and resistance index augment. (4) In 14 rabbits the degrees of carotid stenosis were greater than 50 percent; In 6 animals the injured carotid arteries were occluded because of thrombosis or calcifiation. 3. Carotid digital subtraction angiography: It was showed that vascular walls were not smooth and vascular lemma became centripetally or excentriclly stenotic; There was bad developing in the distal arteries. 4. The degree of carotid stenosis was apparently reduced after CEA, but restenosis take placed in two cases 3 months later. Conclusions: 1. Only injuring the carotid endothelial cells can replicate carotid stenotic rabbits; 2. The carotid stenosis can be assessed by colourful duplex sonography and selective carotid digital subtraction angiography; 3 .CEA can reduce carotid stenosis efficiently, but restenosis may take place later.