Researches On The GC Level In The Serum And GR In The Leukocyte Of Psoriatic Patients

Psoriasis is a common, chronic, recurrent, inflammatory disease of the skin. The occurrence of psoriasis is mainly caused by factors of heredity and enviroment, which associated with dysregulated mind, nervous, immune, and endocrine system, but the exact mechanism of psoriasis is still unknown. Some researches indicate that psoriasis is a kind of psychosomatic disease and is often induced or aggravated by emotional stress in recent years. The stress can decrease the number of GR in the body, which prevent GC from anti-inflammatory and induce or aggravate some inflammatory diseases. Hypothalamic-pituitary-adrenal axis is the main part of the stress system. CRH, ACTH and GC, which are secreted by FIPA axis, play important roles in stress reaction. It is necessary to study the changes of these hormones in psoriasis patients for exploring psychology, mind, nervous, endocrine, immune mechanism ofpsoriasis. AIM:To study the change of GC level in the serum of psoriasis patient before and after treatment and the change of GR mRNA expression in leukocyte of psoriasis patient and investigate the roles of GC and GR in the occurrence of psoriasis. Methods: 1. To detect the GC level in the serum by enzyme immunoassay kit.The serum samples were obtained from 22 psoriatic patients, 15 patients who had been clinical cured and 20 normal controls. There were standard, control, and sample wells. The standardN control and serum samples were pipeted into the appropriate wells and then the enzyme conjugate solution, GC antiserum were pipeted into each wells, incubated the wells at room temperature for 45 minutes, washed and dried each well, added TMB chromogen solution, incubated the wells for 15 minutes, added H2SO4 to stop the reaction. Read the absorbance of the solution in the wells by a microplate reader. Drew the standard curve and determined the GC concentrations of the samples.2. To measured the GR mRNA expression levels in leucocytes by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).Total RNA obtained from peripheral blood leukocytes of 12 psoriasis patients and 10 healthy controls was reverse transcribed, the complementary DNA was amplified using specific primer for GR and GAPDH. The PCR products were separated by electrophoresis in 2% agarose gel and photographed under ultraviolet. Results:The level of serum GC was 20.93?.65 u g 'dL'1 in 22 psoriatic patients, 17.32?.6911 g -dL'1 in 20 normal controls and 17.6313.52 u g ?dL~' in 15 psoriatic patients who had been cured. The level of serum GC was higher in psoriatic patients than that in the normal controls and patients who had been cured(P<0.01). The glucocorticoid receptor mRNA expression level(GR / GAPDH) was 2.26?.18 in blood leukocyte of psoriatic patient and 2.79?.22 in normal control. The glucocorticoid receptor mRNA expression level was significantly lower than that of the normal control (,P<0.01). Conclusion:The glucocorticoid is not lower in psoriatic patients than the normal level; the glucocorticoid receptor mRNA expression level in blood leucocyte of psoriatic patient group is lower than that of the normal control, which suggests there is a dysregulated glucocorticoid, glucocorticoid receptor and corticotropin-releasing hormone system in psoriatic patient, and they may play a role in the pathologic course of psoriasis.