| After craniocerebral trauma, there exist white blood cells and inflammatory cytokines at the damaged part. They have participated in a series of pathological changes after craniocerebral trauma and are closely related to the secondary tissue attack after external cerebral injury. In recent years, researches on endogenous brain-damaging factors and brain -protecting factors have become a hot spot, in which, cytokines have played an important role. By now, cytokines have developed into a huge family that includes interleukins (ILs), chemotactic factors, tumor necrosis factor (TNF), interferon (IFN), colony-stimulating factor (GSF), growth hormone (GH), neurotrophic factors (NTs), neurenergen and etc. The expression of IL-6, IL-1 and TNF-a will obviously increase after craniocerebral trauma. Through interposing and guiding the actions between cells or direct toxic effects, they increase the permeability of blood-brain barrier, promote micro-cerebral thrombosis and lead to the excessive release of central excitatory materials, opening of calcium channel, internal flow of a lot of calcium as well as edema and disintegration of cells. They play an important role in the biochemical dysfunction of nerve cells and secondary craniocerebral trauma after external brain injury. Furthermore, the high-level expression of IL-6 is relatedto nerve restoration. Researches on the expression of TNF-a and IL-6 in serum after external brain injury were started in the 1990s in foreign countries and few relevant researches have been made in China by now. The ABC-ELISA method of double antibody with filling was adopted in this research to check the contents of TNF-a and IL-6 in serum after craniocerebral trauma, compare the relationship between the grading of craniocerebral trauma and the contents of TNF-a and IL-6 in serum, study the trend of changes after external injury and explore its significance.All of the total 31 cases used in this experiment were closed craniocerebral trauma patients that were hospitalized in the Cerebral Surgery Department of Huangyan No. 1 People's Hospital in Sept.~Dec. 2000. According to the Typing of Acute Closed Craniocerebral Trauma amended by China Society of Neurosurgery in 1997, the patients were divided into three groups, including 10 patients with light craniocerebral trauma, 9 patients with moderate craniocerebral trauma and 12 patients with serious craniocerebral trauma. After the patients were hospitalized, all of them received routine cerebral surgical treatments and 3ml of peripheral blood sample was abstracted from each patient respectively within 24 hours, the 3rd day and the 7th day after craniocerebral trauma for separation of serum. Anti-human IL-6 and TNF-a univalent were coated on enzyme labeling board and IL-6 or TNF-a in the sample serum were combined together with the univalent; biotinized anti-human IL-6 or TNF-a was added to form an immune complex that was connected to the board; the streptavidin of horse-radish peroxidase labeling was combined together with biodin; the color changed into yellow after enzyme-substrate OPD was added; the color became deeper after terminating solution sulfuric acid was added; the value of absorbance (OD value) was measured at the point of 492nm; its concentration was in direct proportion with OD value; the concentrations of IL-6 and TNF-a in the samples could be obtained through drawing standard curves. Human TNF-a enzyme linked immunequantitative reagent boxes and human IL-6 enzyme linked immune quantitative reagent boxes were provided by B&C Company. One-Way ANOVA was adopted for testing the contents of IL-6 and TNF-a in the serums of the three groups of craniocerebral trauma patients; Pearson correlation analysis was adopted to compare the three types of craniocerebral trauma; SPSS 9.0 for Windows was used to process all data.It was discovered in this experiment that the contents of TNF-a and IL-6 obviously increased after serious craniocerebral trauma and there were significant differences between the serious craniocerebral... |
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