| The functions of the lower urinary tract are regulated by the coordinated activity of neurons in the brain, spinal cord. The spinal reflex pathways to the bladder can undergo a considerable reorganization following spinal cord injury. Neurocyte nitric oxide synthase inimunoreactivity(ncNOS-IR) is plastic and can be up- regulated by chronic spinal cord injury(SCI). This study was undertaken to examine ncNOS-IR in bladder and to suggest the reorganization mechanism for micturition after SCI. In the meantime, we determine rats bladder ncNOS-IR following intravesical instillation of capsaicin after Sc? Materials and Methods: Adult Sprague-Dawley rats and guinea pigs were randomly divided into normal group, sham-injury group, spinal cord injury group(4?weeks after T~9 spinalization). A dose of 1mM intravesical capsaicin was instilled in part of SCI rats. All animals bladder were divided into three tissue pieces: bladder base, bladder body and bladder dome, which were examined the distribution of ncNOS-IR. Results: Spinal transection induced a significant ncNOS-IR increase in guinea pigs bladder base. In rats, the ncNOS-IR did not differ between the two groups. Instillation of intravesical capsaicin can cause rat bladder ncNOS-IR to be up-regulated significantly by chronic SCI. conclusion: The increase of ncNOS-IR in sci animals bladder indicates that NO may play an important role in the regulation of micturition reflex after SCI. In SCI rats, ncNOS-IR can significantly be up-regulated by intravesical capsaicin instillation, suggesting that NO may act as a factor in the action of capsaicin. Further studies are needed to determine exactly how NO act on urinary bladder.
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