The Synergy Of Prophylactic Administration Of Tumor Necrosis Factor And Verapamil In Repression Of Nude Mice With Human Hepatic Carcinoma

Objective. Tumor Necrosis Factor (TNF) is possessed of a characteristic of cytotoxic effect directly on tumor cells. TNF is limited to be used extensively in clinical setting because of strong side effects itself and lower sensitivity in certain tumor cells. Verapamil (VPL), as a synergist, is employed to seek a way for enhancement of cytotoxic effect and sensitivity of TNF; meanwhile, alleviation its side effects. Another hand, local recurrence of hepatocellular carcinoma always exists certainly after curable operation. It is hoped to approach reasonable and effective method in order to settle this baffling problem. Method. Set up experiment animal groups (BALB/C nude rats): Controlled group; TNF group; VPL group; TNF+VPL group. Inject TNF and/or VPL into ratperitoneal cavity in alternative day for 2 weeks prior to transplant subcutaneously Hep G-2 cells. Observe: tumor growing tendency; microscopic necrosis degree and rang; tumor microvessel density (MVD); and proliferating cells nuclear antigen (PCNA) expression. Result. 1.the repression of growth of hepatic carcinoma could be seen in TNF group; VPL group; and TNF-i-VPL group, the effect is most significant in TNIF+VPL group. 2.Under microscope, tumor necrosis degree and rang is more distinguishable in TNF+VPL group than in TNF group and VPL group. 3. Microvessels count shows MVD is decreased remarkably in TNF+VPL group, compared with TNF group and VPL group (P