| Growth of maignant tumor depends on angiogenesis, the vessels of tumor not onlyprovide tumor with nutrients and remove the catabolites, but also is the derect passage ofmetastases. Angiogenesis is a process through which new blood vessels develop frompre-existing vessels such as capillaries and post-capillary venules. People have beenstudying neovascularization of tumor since 70s this ccentury, and have proved that thisprocess is tightly regulated by a large number of proangiogenic and antiangiogenic factors.Proangiogeneic factors more than 30 kinds have been found, and bFGF(basic fibroblastgrowth factor). VEGF(vascu1ar endothelial growth factor), TGF(transforming growthfactor),and so on, are the several studied more often than not. In the several factors, bFGFis the factor most potently promoting neovascularzation. Antiangiogenic factors morethan 30 kinds have been found, and angioststin,endostatin and IFN(interferon) are themost important in them. IFN is a important kind of cytokines, and prier studies havedetermined its effects of anti-tyumor.In receot investigations, some people have provedthat IFN is a kind of antiangiogenic factor. Rg3 is a kind of monomeric componentextracted from ginseng, and there have been reports about Rg3 inhibiting the growth andmetastases of tunor, but the mechanism has not been completely understanded.In presentstudy, we have investigated the mechanism of IFN and Rg3 inhibiting the growth andmetastases of tumor by measuring their antiangiogenic effects.Objective:l. To invetigate the effects of IFN and Rg3 on tumor-induced angiogenesis and themechanism.2. To investigate whether IFN and Rg3 synergetically inhibit tumor-inducedangiogenesis.Methods:l. Human umbilical vein endothelial cells(HUVEC) and human SPC-A-l lung cancerasMtoar ngcells were cultured in M199 smplemented with l0% fetal bovine serum(FBS) orin 5% FBS, lFN-a and Rg3 were added to the media solution at severalconceniYation, the effects of drugs on cell proliferation was demonstrated bymeasuring the cell proliferation rate with MTT method.2. SPC-A-1 cells were pretreated with IFN or Rg3 of various concentration, and thenthe cultUre media was collected and used in the culture of HUVEC, it wasestimated whether drugs had inhibited the effect of SPC-A-l cells on HUVEC bymeasuring cell proliferation rate.3. IFN or Rg3 were added to various culture media(CM) produced during thecultured of SPC-A-l cells, and then HUVEC were cultured in CM, it wasestimated whether drugs had blocked the effect of CM on HUVEC by measuringcell proliferation rate.4. HUVECs were inoculated in co11egen gel and SPC-A-l cel1 were inoculated on it,and the co-culture was performed with Ml99 containing IFN-a or Rg3 of varyingconcelltfation, the effects of trigs on tUbe-like stIuctUre formation of EC inducedby CM was investigated.5. SPC-A-l ce1ls were inoculated in l640 with or without IFN-a or Rg3 of varyingconcentration, the expression of bFGF was measured with inununohistochemicalstaining method.6. A angiogenesis model of chick chorioallantoic memdranes was made to estimatethe effects of IFN-a and Rg3 on neovascularization.ResuItS:l. Significant cytostasis was seen in both HUVECs and SPC-A~l when theconcentration of IFN-a was l000u/ml, but IFN-a did not thect the prolifefationof ce11s when the concentration was l00u/ml.2. No significan cytostasis was found when the concentrations of Rg3 were25-200Pg/mI.3. The proliferation rate of HUVEC cultrued in CM produced during the culture ofSPC-A-l pretreated by IFN and Rg3 was significantly lower than that cultured inCM not pretreated.5ffe#onar me4. There was no significan difference between the proliferation rate of HUVECsincubated in CM with IFN-a(l0. l00u/ml) and that of control(without drugs);When HUVECs were incubated the CM with Rg3(50. l00. 200pg/ml), theproliferaion rate of EC was significanly lower than that of control.
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