Induction Of Vitreous Cavity-Associated Immune Deviation(VCAID) And Change Of VCAID During Normal Pregnancy

So-called immune privilege sites are defined as anatomical sites that possess different immune activity from other organs. These sites include eye, brain, liver, some endocrine organs and materal-fetal surface. Recent studies implied that many kinds of antigens that were injected into anterior chamber could induce down-regulation of systemic, cell-mediated immune responses, but keep normal Tc activity and non-complement-fixing antibody response. In 1980s, Streilein began to name this specific phenomenon as anterior chamber-associated immune deviation (ACAID). But it is not clear if this phenomenon exists in vitreous cavity (VC). Our research mainly observed whether VC inoculation of 0.5 %BSA could induce vitreous cavity-associated immune deviation (VCAID) and whether this phenomenon exists during normal pregnancy in rats. Moreover, we observed the structure change of thymus during pregnancy in rats. Our objectives are speculating regulatory factors of VCAID that could be related to pregnancy and providing possible strategies for some eye diseases related to immune response.4 暨南大学硕士学位论文(2000)玻璃体腔相关允疫偏离的诱导及其在正常妊娠期间的变化 This research i皿ludes t啊parts as foll侧s:' hti In讪ction and Expression ofWtreous C皿ity血sociated Immune Deviation in ffets ONecti爬 To e似luate whether bWme serum albumin(B肋)inoculation In VC could elicit the iMuctlon ad expression ofaphenomenon similar to壮X互． Methods 0．5％BSA was imected i血o the VCVC of experlmemal group and 7 d叫s laterthose rats were INectedwlthB肋comPmPlete Freund's aquwlltcompouds0 subcutamously．In the 14th d叫 after the Inoculation 0．5％B趴 was ifljected in eachhmdnd foo中ad and we measured meanthickness of＊theickness foo…刎s within24 hours．The data were analyslzed by suitable statistical means． Resuits The foo…ads ofcomrol groupammals swell appare血ly afterthe imection ofo．5％BSA．That ofexperlme血al group Is suppressed o切lously．The statistical amlysis lmpliesthatthere Isstatlstlcaldlfferemebetweentheset侧 gfouPS Conclusion The VCVC inoculation ofsoluble protein a血lgen－B趴 In rats canDIdlCC lfllDlllflC derMlOll SllllifortOD． M 11 Influence ofNormalPregnmcy on vitreous C删tyMsociated Immune D凹iation in伽 ONecti爬 To observe the Influence of pregna啊 on vitreous c洲fly－associatedJ IMllC Qerlstloll Slid hC ChsllRC OI thVffillS ttuCtufC AnflllZ lloflllsl DfCZflsllCV lfl 5rats.Methods 0.5%BSA was injected into the VC of pregnant rats.7 days laterBSAcomPlete Freund's adjuvant comPounds Was injected subcutaneously and14 days later the rats accepted injection of 0.5% BSA in each hind footPad.Wthin 24 hours the thickness of the foorpads was measured and analyzed withstatistical method. In the late pregnancy thymus was nucleated from the rats andhistological and ultrastructUral change was observed.ResultS ComPared with non-pregnant group, the footPad response of pregnantrats could not suppressed obviously. During normal pregnancy the structure ofthymus changed apparently, too. There are Pykonsis, karyolysis and increasedmitochondrion within thymocytes during pregnancy in rats.Conclusion Pregnancy can abrogate the induction and expression of VCAIDobviously and this perhaps correlates with massive change of central immuneorgan-thymus.