Study On The Technology Of Anther Culture Of Capsicum Annuum L

In this article ,the problems of anther culture of capsicum annuum.l were studied,the protocols included the effect of temperature pretreatment,minimal media and suppelemented components to anther culture, the ploidy identification method,transplant of microspore plantlet were carried out.1.We used the alabastrum which contained most uninucleate period of microspores The cytological development period of microspore is at the uninucleate when the length of the petal was as same as the calyx or a little longer, and 1/3 of the anther was purple,2/3 was white and green.2.Lower temperature pretreatment with 4℃on buds before inoculating and higher temperature pretreatment with 35℃on buds after inoculating werestudied.Higher temperature pretreatment with 35℃for 7 days was suitable to all genotypes.3.Orthogonal tests were designed in order to determine the effects of minimal media and suppelemented components,such as plant hormones ,AgNO3, different kinds of sugars ,active charcoal on calli inductivity,embryogenesis and browning.AgNO3 was effect on overcoming the browning,and 6mg/l AgNO3 was more effective to both genotype,the average value of browning was 0.Active charcoal inhibited calli occurrence greatly.Without addition of active charcoal, calli occurrence was high for both genotypes.With addition of active charcoal, calli occurrence was o.Embryogenesis was effected by minimal media,AgNO3,active charcoal ,plant houmones.AgNO3 was in favor of overcoming the browning and increase the embryogenesis,6mg/l AgNO3 was more effective;0.04%~0.1% active charcoal was suitable for both genotypes;when plant houmones group were KT1+NAA0.25, KT0.5+2,4-D0.25,the embryogenesis was high; minimal media NTh was beneficial to get more embryogenesis, minimal media CP R1 was beneficial to get embryogenesis early.The effects of genotypes on the anther culture were obvious.578 could produce much more embryos than 186.Two optimized culture media which were suitable to the two genotypes respectively were found,and a medium suitable to both genotypes was found.4.The procedures of transplantation of anther culture plantlets were established in pepper.The procedures was as follows:hardening off in culture flask→hardening off out of culture flask→transplantation. The surviral rate of anther culture plant1ets could be increased to 85%.growing with ground substance made up of 4 times of humus and one time of vermiculite.The survived plants was with flourishing and luxuriant leaves and developed roots.5.T he ploidy identification method were established in pepper.Put the root tip in the p-Dichlorobenzene solution for 3.5h, then in Carony,s Fluid for 2h,washed by distilled water,dealed with 0.2MHcl for 5min ,washed by distilled water,dealed with 45% glacial acetic acid for 5min ,then dealed with Carblo fuchsine for 20min.