| S-adenosyl-L-methionine(s-adenosyl-L-methionine,as SAM),is important metabolic intermediate which widely exists in animals, plants and microorganisms. It participates in more than 40 kinds of biochemical reactions ,and playing an important role on physiological metabolism metabolism in organism . There are vast market for SAM as nutrient and drug. SAM of this paper have carried out a fermentation with high cell density as well as testing methods and effects on immunity and anti-stress. Results are as follows: The examine method of paper built-up,diving light intensity with Ultraviolet ray and HPLC with Methanol- buffer (obtained 1-Octanesulfonic acid sodium salt monohydrate 1.og and Ammonium formate 5.8g in1000 ml water, formic acid to adjust the value of p H 3.0) for fluid liquid mutually method were built up .The fermentation content of SAM is 250mg / L.Single-factor experiment has biult to optimize the fermentation conditions, the best fermentation medium is KH2PO4 2, ZnSO4.7H20 0.1, MgSO4.7H20 0.1 K2HPO4 1, (NH4) 2SO4 4, sodium citrate 5, MnSO40.3, CaC120.1, glucose 30, L-methionine 3, yeast extract 1(g/L).It were studied and identified the best initial PH is 5.5;best fermentation temperature is 28℃, the best rotation speed is 160r/min, best medium volume 25ml/250ml, best inoculum is 10%.After optimized, SAM-batch production is 250mg / L up to 904mg/mL.Litopenaeus vannamei ,which under the stress response is caused by capture and transport were alloted into seven groups randomly,the first is control group,the three others were the treated groups. The control group was injected with sterile double distilled water, the treated groups were respectively injected with the SAM injection whose concentrations is 1mg/mL, 0.5mg/mL, 0.1mg/mL.The number of dead Litopenaeus vannamei were recorded and determined the immunity after 5,9,12,24,30h.The results are as follows: death ratio: 0.5mg/mL treated group <1mg/mL treated group <0.1mg/mL treated group 5‰the treated group, 10‰control group> 10‰the treated group, 15‰control group> 15‰the treated group. It can be seen that SAM lower death ratio of Litopenaeus vannamei. Compare control group of Litopenaeus vannamei with treated group of Litopenaeus vannamei,the value of NOS, PO, Blood corpuscle number, Hemocynin were significant difference (P <0.05). Blood corpuscle number of Litopenaeus vannamei in control group was significant lower than the treated group (P <0.05), and in downward trend. Blood corpuscle number of treated group is in upward trend between 0 ~ 10h (P <0.05),Blood corpuscle number gradually decreased amang 10 ~ 48h, and were significantly different compared with the control group at the same time poiont( P <0.05), after 48h .it is gradually stabilizing.and higher than the control group. NOS value of Litopenaeus vannamei blood in control group was firstly increased and then decreased.It's up to the highest poiont at 12h, Compared to the treated group, NOS value was significant differentce (P <0.05) and much higher than the treated group. NOS value started to decline amang 24h ~ 48h, stabilized after 48h.(P> 0.05), and significant lower than pre-test. NOS value of Litopenaeus vannamei blood in treated groud was in droping trend amang 0 ~ 24h, 24 ~ 48h,it in rising trend, the value of was stabilizing after 48 hours ,at the same time poiont, value of the control group there was significant differences compared with the NOS value of the control group (P <0.05).In 0 ~ 24h, control group's hemocyanin was significant higher than the corresponding treated group, but significant lower than the treated group amang36 ~ 72h. The control group's hemocyanin was significantly increase firetly in 0 ~ 10h (P <0.05), 10h started to decline, and gradually near to pre-test ; Hemocyanin of treated group was in rising trend between 0 ~ 36h (P <0.05), after 36h ,it jioned in a slow downward trend, and significant differentce at the same time points (P <0.05). PO value of Litopenaeus vannamei injected SAM in the treated group increased first ly and then declined, reached the peak at 24h, and not significant differences between treated groups (P> 0.05). PO values of the treated group bigger than pre-test before 72h, at 72h, closed to pre-test. PO value of Litopenaeus vannamei in control group is rising between 0h ~ 36h, drop to a minimum value at 36 hours, it began to rise after 36h, closed to pre-test at 72h. By single factor analysis of variance,it can be know that the PO value was significant differences between the control group and experimental group (P <0.05),at 72h,it was no significant difference in treated group and control group.(P> 0.05), and all close to the pre-test. Considering death ratio and changes of Immunology index,it can be drawn on SAM has a protective effec on Salinity stress。...
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