Studies On The Descurainia Seed Oil, Flavonoids Extraction And Activity

Taking the descurainia sophia seeds as the study object and adopting the N\natural pharmaceutical chemistry as the method, this paper studies the extraction and activeness of the fat, flavonoids in the descurainia sophia seeds. the following issues are included in the study: study progress of the descurainia sophia, extraction optimization of the total flavone in the descurainia sophia seeds and their pharmacological effects and tests on the mouse's memory.Affilated to the cruciferae descurainia, the descurainia sophia's dry ripe seeds are traditionally called descurainiae, the A-class semen lepidii in the traditional chinese medicine which relieves cough, asthma and detumescence, and is good for urine, strengthens hearts. The descurainia sophia seeds are rich in flavone, sitosterin, cardiac glycoside, isothiocyanates, fat and oils and derivative of butylene sulfide, in which the flavonoids are instrumental in antioxidation, reducing blood pressure, blood sugar and blood fats. The fatty oil in the descurainia sophia seeds consist of fatty acids such as cetylic acid, oleic acid and alpha-linolenic that are helpful in improving the memory, empowering the brain, protecting the eyesight, resisting cancers, reducing blood fats and resisting aging.Extraction of the total flavone: measure the total flavone in the oil cake of the descurainia sophia seeds by means of colorimetry. First, analyze and confirm the main factors affecting the flavone extraction rate through the single-factor experiment, observe the ethanol concentration, solid-to-liquid ratio, ultralsonic time and temperature, four main factors affecting the extraction of the flavone ethanol in the oil cake of the descurainia sophia seeds by orthogonal test. As a result, the extraction of the flavone is taken as the observation index, the factors affecting the total flavone extraction fromthe oil cake of the descurainia sophia seeds are as follows: ethanol concentration > ultrasonic temperature > solid-to-liquid ratio> ultrasonic time and the best conditions for extraction is: 60% ethanol, 60℃of temperature, 20 times of extract liquid and 20 minutes of ultrasonic time. Pharmacodynamic test on the descurainia sophia seeds'improvement of the mouse's memory: intragastric administration of the descurainia seed oil in the mouse with 1.3g/kg,2.6g/kg,5.2g/kg respectively for 15 consecutive days, during which the last time is dealt with molding so as to record the mistaken times of the mouse's jumping and passive avoidance by diving apparatus and passive avoidance apparatus. The study shows that descurainia seed oil can do against the scopolamine hydrobromide resulting in the acquired disability in the mouse's memory which reduces the he mistaken times of the mouse's jumping and passive avoidance.Pharmacological test on the descurainia sophia seeds'improvement of the mouse's memory: take the mouse brain tissues from the pharmacodynamic test as the materials, adopt the colorimetry to measure the ACh in the brain tissues while using kit to measure the activeness of the ACh and and AChE. Finally, the result shows that the activeness of the ACh and CHAT are obviously increased while the activeness of AChE from the brain tissue is reduced.Descurainia oil tasting experiment on human. Self-control design using Comparison of the experimental design group.Male and female subjects were randomly divided into the low dose group, And high dose group. N = 20. Evenly divided between men and women. With the Wechsler Memory Scale test method. Content of the tests measured before and after eating a test. Include distant memory, recent memory and immediate memory three kinds of memory test. The results show that,Subjects taking Descurainia oil soft capsule,Improvement in learning and memory.