Study Of Changes Of Related Enzyme Activity And Endogenous Hormone During Cabbage Veranlization

Head cabbage named cabbage belongs to two year growth plant with green vernaliztion property. Cabbage is honorned as one good eppitizer with its richness of Vitimin K1 and U which are used to resistant stomach ulcer and repair the mucosa-associated lymphoid tissue. Therefore, cabbage is strongly recommended by FAO and fasinated by consumers worldwide.Due to the effect of low temperature, the cabbage prematured lead to serious cabbage quality decrease. So,to study the cabbage vernaliztion mechnanism is the first steop to develop one proper solution by solving this problem. This mechnanism was analysed by measuring the protein, enzyme activity and hermone dynamics during low tempeture vernaliztion, high tempeture vernaliztion and revernalization,Discuss the relation between cabbage vernalization. The results were revealed as follows:In this study, the protein contents, NR activity increased during low tempeture vernalization. When the cabbage seedlings entered bud differentiation by changing from nutritional stage to reproduction stage, it completed vernalization and the protein contents, NR activity surged increasingly and reached the maximum. After vernalization reversion, the protein contents and NR activity decreased sharply and the scale was little with the extention of treating time while the control group increase all the time. Besides, the the protein contents and NR activity while revernalization showed the similar trend as the primary vernalization process. Thus, the protein content and NR activity were benefit to vernalization initiation.In this study, the POD, CAT and EST activity increased during low tempeture vernalization. When the cabbage seedlings completed vernalization, POD, CAT and EST activity decreased sharply. After vernalization reversion, the POD, CAT and EST activity decreased sharply and the scale was little with the extention of treating time while the control group increase all the time. Besides, POD, CAT and EST activity while revernalization showed the similar trend as the primary vernalization process. Thus, the POD, CAT and EST activity were the interior inducer leading cabbage to change from nutritional stage to reproduction stage.In this study, the GA₃ and ABA contents increased during low tempeture vernalization. When the cabbage seedlings completed vernalization by enterring bud differentiation stage, GA₃ and ABA contents surged while the IAA decreased. After vernalization reversion, the GA₃ and ABA contents decreased whereas the IAA contents increased and the scale was little with the extention of treating time. The control group demonstrated increasing and dereasing phenomena all the time. Besides, GA, ABA and IAA contents while revernalization showed the similar trend as the primary vernalization process. Thus, the GA₃, ABA and IAA had benefits to cabbage vernalization initiation.Protein, enzyme activity and hormone effect the cabbage vernalization, no whether protein, enzyme activity or hormone have the same tendency, but different variety have the different level of them. Be embodied as follow: cabbage plants subjected to low temperature during vernalization showed increased protein, enzyme activity and ABA, after termination of vernalization the cabbage plants developed the highest protein, enzyme activity and ABA, while GA₃ decreased drastically. There is a contrast performance for IAA in the whole process of vernalization and revernalization;in reaction to the high temperature there is a strong decrease in protein, enzyme activity, GA₃ and ABA during devernalization,but IAA increased; the not-devernalization plants, after high temperature treatment there appears small change in protein, enzyme activity, GA₃ and ABA.