Screening Of Disinfector And Research Of Codes For Disinfection In Air Of Large-scale Laying House

The aim:By testing bacteriostasis and disinfection capability of 15 kinds of disinfector-Didecyl dimenthl ammonium bromide solution10%, Dilute glutaral solution, 5% Povidohe Iodine, H2O2,84, Hydrocarbon dinethy ammonium chloride compound glutaral soluation, Benzalkonium bromide solution,20g/L Povidone Iodine solution, For-maldehyde solution, Bromogeramine,45% complex phenol,3% Povidone Iodine,41 %complex phenol, Weak acid hypochlorous water, Electrolyzed oxidizing water-we could screen out disinfector of strong bacteriostasis and disinfection capability. Then we set up Codes for disinfenction of large scale laying house in summer and winter by using screened disinfector, design perpendicular analysis for factor of disinfective effectivenes-s, choose optimal combination and institute effective regulation which is most suited for large scale laying house. The method:Using broth dilution method to test the minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) of 15 kinds of disinfector toward standard strain and strain from laying house, using suspension quantitation bactericidal trial to test disinfective effectiveness of 15 kinds of infector toward 3 kinds of standard strain, using natural sedimentation to test total viable organism of large scale of laying house and using orthogonal experiment to analyze and research the effectiveness of each factor toward disinfective effectiveness.The result:Using analysis by synthesis of 15 kinds of disinfector MIC, MBC and disinfective effectiveness, we could screen out disinfector of strong disinfection and bacteriostasis capability, such as Didecyl dimenthl ammonium bromide solution10%, Weak acid hypochlorous water, Electrolyzed oxidizing water, Dilute glutaral solution, Bromogeramine, Hydrocarbon dinethy ammonium chloride compound glutaral soluatio -n,3% Povidone Iodine. Advice for disinfector program in summer:Using 312.5mg/L Didecyl dimenthl ammonium bromide solution 10% in non-laying house to spray 50ml/m3 and disinfect once every 108h. Using 625mg/L Didecyl dimenthl ammonium bromide solution10% in laying house to spray 30ml/m3 and disinfect once every 84h. Advice for disinfector program in winter:Using 625mg/L Didecyl dimenthl ammonium bromide solution 10% in laying house to spray 18ml/m3 and disinfect once every 66h. Using analysis of range and analysis of variance for factor of disinfective effectiveness, we could find that the influence of the kinds and concentration of disinfector toward disinfective effectiveness in summer design of experimentis great significant (P<0.01). But the influnce of the spraying amount toward disinfective effectiveness is not significant (P>0.05). The optimal combination of disinfective program is that we should dilut Didecyl dimenthl ammonium bromide solution 10% to 625mg/L, spray 50ml/m3 and disinfect once every 90h. Furthermore, we could find that the influence of spraying amount toward disinfective effectiveness in winter design of experiment is significant (P<0.05), the influnce of the concentration and the kinds of disinfector toward disinfective effectiveness is much significant (P<0.01) and the influnce of disinfective time toward disinfective effectiveness is not significant (P>0.05).The optimal combination of disinfective program is that we should dilut Didecyl dimenthl ammonium bromide solution 10% to 625mg/L, spray 24ml/m3, disinfect by 30 min and disinfect every 72h.