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Effect Of Urea Treated Leuecana Leueoecphela Leaf Meal On Rumen Fermentation And Growth Performance Of Growing Nanjiang Goat

Posted on:2011-10-14Degree:MasterType:Thesis
Country:ChinaCandidate:Y H YangFull Text:PDF
GTID:2143360308972244Subject:Animal Nutrition and Feed Science
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Abstract:In this study, four tests were conducted. The first test was conducted to study the urea ammoniating treatment on the influence of the content of mimosine and nutrient in the leucaena leucocephala leaf meal (L). In vitro gas production technology was used to investigate the fermentation characteristics of leucaena leucocephala leaf meal ammoniated with different level of urea (TL) and L during the second test. Then the third test was performed to evaluate three different diets which incorporation with 20% TL or L into the concentrates or not on the effect of in vitro fermentation characteristics, and the last test was carried out to study the effect of TL on the growth performance and blood parameters of Nanjiang goats.In experiment 1, leucaena leucocephala leaf meal (L) was naturally dried, or ammoniated with 4%,8% and 15% urea to study the content change of mimosine,2, 3-DHP and the nutrient. The results showed that the contents of mimosine and 2,3-DHP were separately degraded or increased significantly with the urea level increase (P<0.01). Meanwhile, the contents of CP and NDF were separately significantly increased or redused in TL compared to L (P<0.05), and the concentrates of ADF and EE were not changed significantly (P>0.05). However, the NDF content of 4% urea TL didn't decreased much (P>0.05), comparing to the L.In experiment 2, leucaena leucocephala leaf meal naturally dried (L) or treated with 4%, 8% and 15% of urea were used to compare the in vitro gas production volum at 2 to 48 hr, the pH value and NH3-N concentrate at 48 hr time point. The results showed that the gas production volum of TL was decreased, compared to L (P<0.01), and decreased significantly with the urea level increase (P<0.01). The reduction of the gas production parameters was aggravated with urea level increase (P<0.01). L ammoniated with different level of urea significantly raised the pH value at 48 hr, simultaneity the concentrate of NH3-H was also increased (P<0.01). However, the change of pH value and NH3-H content were not beyond the normal level.In experiment 3, three different diets which contained the concentrate adding 20% TL ammoniated with 4% urea or L to partially substitute the regular concentrate feed was used to investigate the effect on the in vitro fermentation and the three diets were allotted to urea treated group natural dried group and control group separately. The result showed that urea treated group significantly increased the gas production at 2~48 hr (P<0.01), and buffered the change of pH value in the fermentation fluid, and the general trend of NH3-H concentrate was decreased and appeared lowly with different degree by contrast with the other two groups at 6hr,8hr,24hr,48hr. The production of acetate, propionate, butyrate and total VFA was improved after 6hr with different degree, while the ratio of acetate to propionate were not differed among groups (P>0.05).In experiment 4, fifteen castrated Nanjiang goats (20.38kg±1.51kg; 6 months of age) were allotted into three treatments according to their BW with 5 replicates per treatment and 1 goat per replicate. The treatments were allotted to feeding different diets, and the diets were same to the experiment 3. The experiment lasted for 28 days, the lasted 4 days were used to examine digestibility. The result showed that all goats fed with TLL and ULL did not express any toxic symptom. Average daily gain in TLL group was higher than other groups (P=0.01), and had a lower feed to gain ratio. Apparent digestibility of organic matter, crude protein and NDF in TLL group had a trend to be higher than CON and ULL (P>0.05), especially, ADF was significantly higher in TLL (P<0.05). Hematologica parameters varied in normal physiological range and there were no obvious difference among the groups (P>0.05). There was no difference of BUN, TP, GOT, GPT, T3 and T4 among each treatment groups (P>0.05), but ALB in TLL was higher compared with the other two groups (P<0.05).Collectively, urea ammoniation was an efficiency technology to reduce mimosine in leucaena leucocephala leaf meal, which could improve the content of CP and decrease the content of NDF. Singly using the urea ammoniated leucaena leucocephala leaf meal was not benifical to the nutrient degradation and utilization, however, when it incorporated with other feed could impove the ruminal fermentation characteristics. Incorporation of urea treated leucaena leucocephala leaf meal is a profitably methods to improve the in vitro ruminal fermentation characteristics and the nutrients digestibility, protein utilization, growth performance of Nanjiang goat, and also with no obvious affect on blood parameters and the secretion of T3 and T4.
Keywords/Search Tags:ammoniating leucaena leucocephala leaf meal, mimosine, rumen fermentation, growing performance, nanjiang goat
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